1p35

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[[Image:1p35.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF BACULOVIRUS P35==
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The line below this paragraph, containing "STRUCTURE_1p35", creates the "Structure Box" on the page.
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<StructureSection load='1p35' size='340' side='right'caption='[[1p35]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p35]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P35 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1p35| PDB=1p35 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p35 OCA], [https://pdbe.org/1p35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p35 RCSB], [https://www.ebi.ac.uk/pdbsum/1p35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p35 ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF BACULOVIRUS P35'''
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== Function ==
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[https://www.uniprot.org/uniprot/P35_NPVAC P35_NPVAC] Functions as an inhibitor of the host RNA interference antiviral response. Inhibits the insect host cell apoptotic response initiated by the viral infection. Blocks as well the activity of members of the caspase family of proteases. Required for late and very late gene expression.<ref>PMID:16081248</ref> <ref>PMID:1962198</ref> <ref>PMID:26018163</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The aspartate-specific caspases are critical protease effectors of programmed cell death and consequently represent important targets for apoptotic intervention. Baculovirus P35 is a potent substrate inhibitor of metazoan caspases, a property that accounts for its unique effectiveness in preventing apoptosis in phylogenetically diverse organisms. Here we report the 2.2 A resolution crystal structure of P35, the first structure of a protein inhibitor of the death caspases. The P35 monomer possesses a solvent-exposed loop that projects from the protein's main beta-sheet core and positions the requisite aspartate cleavage site at the loop's apex. Distortion or destabilization of this reactive site loop by site-directed mutagenesis converted P35 to an efficient substrate which, unlike wild-type P35, failed to interact stably with the target caspase or block protease activity. Thus, cleavage alone is insufficient for caspase inhibition. These data are consistent with a new model wherein the P35 reactive site loop participates in a unique multi-step mechanism in which the spatial orientation of the loop with respect to the P35 core determines post-cleavage association and stoichiometric inhibition of target caspases.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/1p35_consurf.spt"</scriptWhenChecked>
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1P35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P35 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of baculovirus P35: role of a novel reactive site loop in apoptotic caspase inhibition., Fisher AJ, Cruz W, Zoog SJ, Schneider CL, Friesen PD, EMBO J. 1999 Apr 15;18(8):2031-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10205157 10205157]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p35 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Autographa californica nucleopolyhedrovirus]]
[[Category: Autographa californica nucleopolyhedrovirus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Delacruz, W P.]]
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[[Category: Delacruz WP]]
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[[Category: Fisher, A J.]]
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[[Category: Fisher AJ]]
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[[Category: Friesen, P D.]]
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[[Category: Friesen PD]]
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[[Category: Schneider, C L.]]
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[[Category: Schneider CL]]
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[[Category: Zoog, S J.]]
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[[Category: Zoog SJ]]
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[[Category: Apoptosis]]
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[[Category: Baculovirus]]
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[[Category: Cell death]]
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[[Category: P35]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:37:26 2008''
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Current revision

CRYSTAL STRUCTURE OF BACULOVIRUS P35

PDB ID 1p35

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