1p5o
From Proteopedia
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| - | [[Image:1p5o.gif|left|200px]] | ||
| - | + | ==Solution Structure of HCV IRES Domain II== | |
| - | + | <StructureSection load='1p5o' size='340' side='right'caption='[[1p5o]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1p5o]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P5O FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p5o OCA], [https://pdbe.org/1p5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p5o RCSB], [https://www.ebi.ac.uk/pdbsum/1p5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p5o ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | '' | + | == Publication Abstract from PubMed == |
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Complex RNA structures regulate many biological processes, but are often too large for structure determination by NMR methods. The 5' untranslated region (5' UTR) of the hepatitis C viral (HCV) RNA genome contains an internal ribosome entry site (IRES) that binds to 40S ribosomal subunits with high affinity and specificity to control translation. Domain II of the HCV IRES forms a 25-kDa folded subdomain that may alter ribosome conformation. We report here the structure of domain II as determined using an NMR approach that combines short- and long-range structural data. Domain II adopts a distorted L-shape structure, and its overall shape in the free form is markedly similar to its 40S subunit-bound form; this suggests how domain II may modulate 40S subunit conformation. The results show how NMR can be used for structural analysis of large biological RNAs. | Complex RNA structures regulate many biological processes, but are often too large for structure determination by NMR methods. The 5' untranslated region (5' UTR) of the hepatitis C viral (HCV) RNA genome contains an internal ribosome entry site (IRES) that binds to 40S ribosomal subunits with high affinity and specificity to control translation. Domain II of the HCV IRES forms a 25-kDa folded subdomain that may alter ribosome conformation. We report here the structure of domain II as determined using an NMR approach that combines short- and long-range structural data. Domain II adopts a distorted L-shape structure, and its overall shape in the free form is markedly similar to its 40S subunit-bound form; this suggests how domain II may modulate 40S subunit conformation. The results show how NMR can be used for structural analysis of large biological RNAs. | ||
| - | + | Structure of HCV IRES domain II determined by NMR.,Lukavsky PJ, Kim I, Otto GA, Puglisi JD Nat Struct Biol. 2003 Dec;10(12):1033-8. Epub 2003 Oct 26. PMID:14578934<ref>PMID:14578934</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1p5o" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Kim I]] |
| - | [[Category: | + | [[Category: Lukavsky PJ]] |
| - | [[Category: | + | [[Category: Otto GA]] |
| - | [[Category: | + | [[Category: Puglisi JD]] |
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Current revision
Solution Structure of HCV IRES Domain II
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