1p6v

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[[Image:1p6v.gif|left|200px]]
 
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==Crystal structure of the tRNA domain of transfer-messenger RNA in complex with SmpB==
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The line below this paragraph, containing "STRUCTURE_1p6v", creates the "Structure Box" on the page.
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<StructureSection load='1p6v' size='340' side='right'caption='[[1p6v]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p6v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P6V FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p6v OCA], [https://pdbe.org/1p6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p6v RCSB], [https://www.ebi.ac.uk/pdbsum/1p6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p6v ProSAT]</span></td></tr>
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{{STRUCTURE_1p6v| PDB=1p6v | SCENE= }}
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</table>
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== Function ==
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'''Crystal structure of the tRNA domain of transfer-messenger RNA in complex with SmpB'''
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[https://www.uniprot.org/uniprot/SSRP_AQUAE SSRP_AQUAE] Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Accurate translation of genetic information into protein sequence depends on complete messenger RNA molecules. Truncated mRNAs cause synthesis of defective proteins, and arrest ribosomes at the end of their incomplete message. In bacteria, a hybrid RNA molecule that combines the functions of both transfer and messenger RNAs (called tmRNA) rescues stalled ribosomes, and targets aberrant, partially synthesized, proteins for proteolytic degradation. Here we report the 3.2-A-resolution structure of the tRNA-like domain of tmRNA (tmRNA(Delta)) in complex with small protein B (SmpB), a protein essential for biological functions of tmRNA. We find that the flexible RNA molecule adopts an open L-shaped conformation and SmpB binds to its elbow region, stabilizing the single-stranded D-loop in an extended conformation. The most striking feature of the structure of tmRNA(Delta) is a 90 degrees rotation of the TPsiC-arm around the helical axis. Owing to this unusual conformation, the SmpB-tmRNA(Delta) complex positioned into the A-site of the ribosome orients SmpB towards the small ribosomal subunit, and directs tmRNA towards the elongation-factor binding region of the ribosome. On the basis of this structure, we propose a model for the binding of tmRNA on the ribosome.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/1p6v_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1P6V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6V OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p6v ConSurf].
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Crystal structure of the transfer-RNA domain of transfer-messenger RNA in complex with SmpB., Gutmann S, Haebel PW, Metzinger L, Sutter M, Felden B, Ban N, Nature. 2003 Aug 7;424(6949):699-703. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12904796 12904796]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ban, N.]]
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[[Category: Ban N]]
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[[Category: Felden, B.]]
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[[Category: Felden B]]
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[[Category: Gutmann, S.]]
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[[Category: Gutmann S]]
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[[Category: Haebel, P W.]]
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[[Category: Haebel PW]]
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[[Category: Metzinger, L.]]
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[[Category: Metzinger L]]
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[[Category: Sutter, M.]]
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[[Category: Sutter M]]
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[[Category: Protein-rna complex]]
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[[Category: Smpb]]
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[[Category: Tmrna]]
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[[Category: Trans-translation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:45:37 2008''
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Current revision

Crystal structure of the tRNA domain of transfer-messenger RNA in complex with SmpB

PDB ID 1p6v

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