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| - | [[Image:1p9i.jpg|left|200px]] | |
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| - | <!--
| + | ==Coiled-coil X-ray structure at 1.17 A resolution== |
| - | The line below this paragraph, containing "STRUCTURE_1p9i", creates the "Structure Box" on the page.
| + | <StructureSection load='1p9i' size='340' side='right'caption='[[1p9i]], [[Resolution|resolution]] 1.17Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1p9i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9I FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.17Å</td></tr> |
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| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9i OCA], [https://pdbe.org/1p9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9i RCSB], [https://www.ebi.ac.uk/pdbsum/1p9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9i ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1p9i| PDB=1p9i | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''Coiled-coil X-ray structure at 1.17 A resolution'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Ivaninskii S]] |
| - | ==Overview==
| + | |
| - | We determined the 1.17 A resolution X-ray crystal structure of a hybrid peptide based on sequences from coiled-coil regions of the proteins GCN4 and cortexillin I. The peptide forms a parallel homodimeric coiled-coil, with C(alpha) backbone geometry similar to GCN4 (rmsd value 0.71 A). Three stabilizing interactions have been identified: a unique hydrogen bonding-electrostatic network not previously observed in coiled-coils, and two other hydrophobic interactions involving leucine residues at positions e and g from both g-a' and d-e' interchain interactions with the hydrophobic core. This is also the first report of the quantitative significance of these interactions. The GCN4/cortexillin hybrid surprisingly has two interchain Glu-Lys' ion pairs that form a hydrogen bonding network with the Asn residues in the core. This network, which was not observed for the reversed Lys-Glu' pair in GCN4, increases the combined stability contribution of each Glu-Lys' salt bridge across the central Asn15-Asn15' core to approximately 0.7 kcal/mole, compared to approximately 0.4 kcal mole(-1) from a Glu-Lys' salt bridge on its own. In addition to electrostatic and hydrogen bonding stabilization of the coiled-coil, individual leucine residues at positions e and g in the hybrid peptide also contribute to stability by 0.7 kcal/mole relative to alanine. These interactions are of critical importance to understanding the stability requirements for coiled-coil folding and in modulating the stability of de novo designed macromolecules containing this motif.
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| - | | + | |
| - | ==About this Structure== | + | |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9I OCA]. | + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Unique stabilizing interactions identified in the two-stranded alpha-helical coiled-coil: crystal structure of a cortexillin I/GCN4 hybrid coiled-coil peptide., Lee DL, Ivaninskii S, Burkhard P, Hodges RS, Protein Sci. 2003 Jul;12(7):1395-405. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12824486 12824486]
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| - | [[Category: Ivaninskii, S.]] | + | |
| - | [[Category: Coiled-coil]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:51:05 2008'' | + | |
