1p9p

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:06, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1p9p.gif|left|200px]]
 
-
<!--
+
==The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD==
-
The line below this paragraph, containing "STRUCTURE_1p9p", creates the "Structure Box" on the page.
+
<StructureSection load='1p9p' size='340' side='right'caption='[[1p9p]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1p9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9P FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-
{{STRUCTURE_1p9p| PDB=1p9p | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9p OCA], [https://pdbe.org/1p9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9p RCSB], [https://www.ebi.ac.uk/pdbsum/1p9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9p ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TRMD_ECOLI TRMD_ECOLI] Specifically methylates guanosine-37 in various tRNAs.<ref>PMID:14583191</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9p_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9p ConSurf].
 +
<div style="clear:both"></div>
-
'''The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD'''
+
==See Also==
-
 
+
*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
The crystal structure of Escherichia coli tRNA (guanosine-1) methyltransferase (TrmD) complexed with S-adenosyl homocysteine (AdoHcy) has been determined at 2.5A resolution. TrmD, which methylates G37 of tRNAs containing the sequence G36pG37, is a homo-dimer. Each monomer consists of a C-terminal domain connected by a flexible linker to an N-terminal AdoMet-binding domain. The two bound AdoHcy moieties are buried at the bottom of deep clefts. The dimer structure appears integral to the formation of the catalytic center of the enzyme and this arrangement strongly suggests that the anticodon loop of tRNA fits into one of these clefts for methyl transfer to occur. In addition, adjacent hydrophobic sites in the cleft delineate a defined pocket, which may accommodate the GpG sequence during catalysis. The dimer contains two deep trefoil peptide knots and a peptide loop extending from each knot embraces the AdoHcy adenine ring. Mutational analyses demonstrate that the knot is important for AdoMet binding and catalytic activity, and that the C-terminal domain is not only required for tRNA binding but plays a functional role in catalytic activity.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Escherichia coli K-12]]
-
1P9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli,_escherichia_coli_o6,_escherichia_coli_o157:h7,_and_shigella_flexneri Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9P OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Andraos-Selim C]]
-
==Reference==
+
[[Category: Elkins PA]]
-
Insights into catalysis by a knotted TrmD tRNA methyltransferase., Elkins PA, Watts JM, Zalacain M, van Thiel A, Vitazka PR, Redlak M, Andraos-Selim C, Rastinejad F, Holmes WM, J Mol Biol. 2003 Nov 7;333(5):931-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14583191 14583191]
+
[[Category: Holmes WM]]
-
[[Category: Escherichia coli, escherichia coli o6, escherichia coli o157:h7, and shigella flexneri]]
+
[[Category: Rastinejad F]]
-
[[Category: Single protein]]
+
[[Category: Redlak M]]
-
[[Category: Andraos-Selim, C.]]
+
[[Category: Van Thiel A]]
-
[[Category: Elkins, P A.]]
+
[[Category: Vitaszka PR]]
-
[[Category: Holmes, W M.]]
+
[[Category: Watts JM]]
-
[[Category: Rastinejad, F.]]
+
[[Category: Zalacain M]]
-
[[Category: Redlak, M.]]
+
-
[[Category: Thiel, A Van.]]
+
-
[[Category: Vitaszka, P R.]]
+
-
[[Category: Watts, J M.]]
+
-
[[Category: Zalacain, M.]]
+
-
[[Category: Adomet]]
+
-
[[Category: Knot]]
+
-
[[Category: Methyltransferase]]
+
-
[[Category: S-adenosylmethionine]]
+
-
[[Category: Spou]]
+
-
[[Category: Spout]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:51:37 2008''
+

Current revision

The Crystal Structure of a M1G37 tRNA Methyltransferase, TrmD

PDB ID 1p9p

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p9p"
Personal tools