1pbz

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[[Image:1pbz.gif|left|200px]]
 
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==DE NOVO DESIGNED PEPTIDE-METALLOPORPHYRIN COMPLEX, SOLUTION STRUCTURE==
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The line below this paragraph, containing "STRUCTURE_1pbz", creates the "Structure Box" on the page.
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<StructureSection load='1pbz' size='340' side='right'caption='[[1pbz]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pbz]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PBZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 14 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PC3:COPROPORPHYRIN+I+CONTAINING+CO(III)'>PC3</scene></td></tr>
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{{STRUCTURE_1pbz| PDB=1pbz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pbz OCA], [https://pdbe.org/1pbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pbz RCSB], [https://www.ebi.ac.uk/pdbsum/1pbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pbz ProSAT]</span></td></tr>
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</table>
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'''DE NOVO DESIGNED PEPTIDE-METALLOPORPHYRIN COMPLEX, SOLUTION STRUCTURE'''
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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==Overview==
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The structural characterization of de novo designed metalloproteins together with determination of chemical reactivity can provide a detailed understanding of the relationship between protein structure and functional properties. Toward this goal, we have prepared a series of cyclic peptides that bind to water-soluble metalloporphyrins (FeIII and CoIII). Neutral and positively charged histidine-containing peptides bind with a high affinity, whereas anionic peptides bind only weakly to the negatively charged metalloporphyrin. Additionally, it was found that the peptide becomes helical only in the presence of the metalloporphyrin. CD experiments confirm that the metalloporphyrin binds specific cyclic peptides with high affinity and with isodichroic behavior. Thermal unfolding experiments show that the complex has "native-like" properties. Finally, NMR spectroscopy produced well dispersed spectra and experimental restraints that provide a high-resolution solution structure of the complexed peptide.
The structural characterization of de novo designed metalloproteins together with determination of chemical reactivity can provide a detailed understanding of the relationship between protein structure and functional properties. Toward this goal, we have prepared a series of cyclic peptides that bind to water-soluble metalloporphyrins (FeIII and CoIII). Neutral and positively charged histidine-containing peptides bind with a high affinity, whereas anionic peptides bind only weakly to the negatively charged metalloporphyrin. Additionally, it was found that the peptide becomes helical only in the presence of the metalloporphyrin. CD experiments confirm that the metalloporphyrin binds specific cyclic peptides with high affinity and with isodichroic behavior. Thermal unfolding experiments show that the complex has "native-like" properties. Finally, NMR spectroscopy produced well dispersed spectra and experimental restraints that provide a high-resolution solution structure of the complexed peptide.
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==About this Structure==
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De novo designed cyclic-peptide heme complexes.,Rosenblatt MM, Wang J, Suslick KS Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13140-5. Epub 2003 Oct 31. PMID:14595023<ref>PMID:14595023</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PBZ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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De novo designed cyclic-peptide heme complexes., Rosenblatt MM, Wang J, Suslick KS, Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13140-5. Epub 2003 Oct 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14595023 14595023]
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</div>
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[[Category: Rosenblatt, M M.]]
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<div class="pdbe-citations 1pbz" style="background-color:#fffaf0;"></div>
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[[Category: Suslick, K S.]]
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== References ==
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[[Category: Wang, J.]]
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<references/>
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[[Category: De novo design]]
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__TOC__
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[[Category: Heme]]
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</StructureSection>
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[[Category: Metalloporphyrin]]
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[[Category: Large Structures]]
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[[Category: Peptide]]
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[[Category: Rosenblatt MM]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:55:26 2008''
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[[Category: Suslick KS]]
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[[Category: Wang J]]

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DE NOVO DESIGNED PEPTIDE-METALLOPORPHYRIN COMPLEX, SOLUTION STRUCTURE

PDB ID 1pbz

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