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1pc9

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[[Image:1pc9.gif|left|200px]]
 
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==Crystal Structure of BnSP-6, a Lys49-Phospholipase A2==
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The line below this paragraph, containing "STRUCTURE_1pc9", creates the "Structure Box" on the page.
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<StructureSection load='1pc9' size='340' side='right'caption='[[1pc9]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pc9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_pauloensis Bothrops pauloensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PC9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pc9 OCA], [https://pdbe.org/1pc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pc9 RCSB], [https://www.ebi.ac.uk/pdbsum/1pc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pc9 ProSAT]</span></td></tr>
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{{STRUCTURE_1pc9| PDB=1pc9 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA2H_BOTPA PA2H_BOTPA] Snake venom phospholipase A2 (PLA2) that lacks enzymatic activity. Is myotoxic. Displays bactericidal activity and promotes the blockage of the neuromuscular contraction of the chick biventer cervicis muscle. Also disrupts artificial membranes, and provokes tissue damages characterized by edema, necrosis and inflammation. May act as pro-inflammatory mediators, inducing metalloproteinase and cytokine production from the inflammatory and satellite cells.<ref>PMID:10860537</ref> <ref>PMID:9972319</ref> <ref>PMID:14623331</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/1pc9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pc9 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of BnSP-6, a Lys49-Phospholipase A2'''
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==See Also==
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*[[Phospholipase A2 homolog|Phospholipase A2 homolog]]
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== References ==
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==Overview==
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<references/>
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Phospholipases A(2) are components of Bothrops venoms responsible for disruption of cell membrane integrity via hydrolysis of its phospholipids. A class of PLA(2)-like proteins has been described which despite PLA(2) activity on artificial substrate, due to a D49K mutation, is still highly myonecrotic. This work reports the X-ray structure determination of two Lys49-PLA(2)s from Bothrops neuwiedi pauloensis (BnSP-7 and BnSP-6) and, for the first time, the comparison of eight dimeric Lys49-PLA(2)s. This comparison reveals that there are not just two ("open" and "closed") but at least six different conformations. The binding of fatty acid observed in three recent Lys49-PLA(2) structures seems to be independent of their quaternary conformation. Cys29 polarization by Lys122 is not significant for BnSP-7 and BnSP-6 or other structures not bound by fatty acids. These structures may be in an "active" state when nothing is bound to them and the Lys122/Cys29 interactions are weak or absent.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bothrops pauloensis]]
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1PC9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bothrops_neuwiedi_pauloensis Bothrops neuwiedi pauloensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC9 OCA].
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[[Category: Large Structures]]
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[[Category: Fontes MRM]]
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==Reference==
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[[Category: Giglio JR]]
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Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights., Magro AJ, Soares AM, Giglio JR, Fontes MR, Biochem Biophys Res Commun. 2003 Nov 21;311(3):713-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623331 14623331]
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[[Category: Magro AJ]]
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[[Category: Bothrops neuwiedi pauloensis]]
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[[Category: Soares AM]]
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[[Category: Single protein]]
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[[Category: Fontes, M R.M.]]
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[[Category: Giglio, J R.]]
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[[Category: Magro, A J.]]
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[[Category: Soares, A M.]]
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[[Category: Bothrop]]
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[[Category: Crystal structure]]
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[[Category: Lys49-phospholipase a2]]
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[[Category: Myotoxin]]
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[[Category: Venom.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:55:53 2008''
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Current revision

Crystal Structure of BnSP-6, a Lys49-Phospholipase A2

PDB ID 1pc9

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