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| - | [[Image:1pfs.jpg|left|200px]] | |
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| - | <!-- | + | ==SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE== |
| - | The line below this paragraph, containing "STRUCTURE_1pfs", creates the "Structure Box" on the page.
| + | <StructureSection load='1pfs' size='340' side='right'caption='[[1pfs]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1pfs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_Pf3 Pseudomonas phage Pf3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFS FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
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| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfs OCA], [https://pdbe.org/1pfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfs RCSB], [https://www.ebi.ac.uk/pdbsum/1pfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfs ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1pfs| PDB=1pfs | SCENE= }}
| + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G5P_BPPF3 G5P_BPPF3] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity). |
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| - | '''SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE'''
| + | ==See Also== |
| - | | + | *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The three-dimensional structure of the homodimeric single-stranded DNA binding protein encoded by the filamentous Pseudomonas bacteriophage Pf3 has been determined using heteronuclear multidimensional NMR techniques and restrained molecular dynamics. NMR experiments and structure calculations have been performed on a mutant protein (Phe36 --> His) that was successfully designed to reduce the tendency of the protein to aggregate. The protein monomer is composed of a five-stranded antiparallel beta-sheet from which two beta-hairpins and a large loop protrude. The structure is compared with the single-stranded DNA binding protein encoded by the filamentous Escherichia coli phage Ff, a protein with a similar biological function and DNA binding properties, yet quite different amino acid sequence, and with the major cold shock protein of Escherichia coli, a single-stranded DNA binding protein with an entirely different sequence, biological function and binding characteristics. The amino acid sequence of the latter is highly homologous to the nucleic acid binding domain (i.e. the cold shock domain) of proteins belonging to the Y-box family. Despite their differences in amino acid sequence and function, the folds of the three proteins are remarkably similar, suggesting that this is a preferred folding pattern shared by many single-stranded DNA binding proteins.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Pseudomonas phage Pf3]] |
| - | ==About this Structure==
| + | [[Category: Folmer RHA]] |
| - | 1PFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf3 Pseudomonas phage pf3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFS OCA].
| + | [[Category: Hilbers CW]] |
| - | | + | [[Category: Konings RNH]] |
| - | ==Reference==
| + | [[Category: Nilges M]] |
| - | Solution structure of the single-stranded DNA binding protein of the filamentous Pseudomonas phage Pf3: similarity to other proteins binding to single-stranded nucleic acids., Folmer RH, Nilges M, Konings RN, Hilbers CW, EMBO J. 1995 Sep 1;14(17):4132-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7556054 7556054]
| + | |
| - | [[Category: Pseudomonas phage pf3]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Folmer, R H.A.]] | + | |
| - | [[Category: Hilbers, C W.]] | + | |
| - | [[Category: Konings, R N.H.]] | + | |
| - | [[Category: Nilges, M.]] | + | |
| - | [[Category: Bacteriophage pf3]]
| + | |
| - | [[Category: Dna-binding protein]]
| + | |
| - | [[Category: Single-stranded dna]]
| + | |
| - | [[Category: Viral]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:02:12 2008''
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