8rlz
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==NMR solution structure of the N-terminal cytoplasmic domain, DdvANt, of the membrane antisigma factor DdvA== | |
| + | <StructureSection load='8rlz' size='340' side='right'caption='[[8rlz]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8rlz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RLZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rlz OCA], [https://pdbe.org/8rlz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rlz RCSB], [https://www.ebi.ac.uk/pdbsum/8rlz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rlz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | How CRISPR-Cas and cyclic oligonucleotide-based antiphage signaling systems (CBASS) are coordinately deployed against invaders remains unclear. We show that a locus containing two CBASS and one type III-B CRISPR-Cas system, regulated by the transmembrane anti-sigma DdvA and its cognate extracytoplasmic function (ECF) sigma DdvS, can defend Myxococcus xanthus against a phage. Cryo-electron microscopy reveals DdvA-DdvS pairs assemble as arrow-shaped transmembrane dimers. Each DdvA periplasmic domain adopts a separase/craspase-type tetratricopeptide repeat (TPR)-caspase HetF-associated with TPR (TPR-CHAT) architecture with an incomplete His-Cys active site, lacking three alpha-helices conserved among CHAT domains. Each active site faces the dimer interface, raising the possibility that signal-induced caspase-like DdvA autoproteolysis in trans precedes RseP-mediated intramembrane proteolysis and DdvS release. Nuclear magnetic resonance reveals a DdvA cytoplasmic CHCC-type zinc-bound three-helix bundle that binds to DdvS sigma(2) and sigma(4) domains, undergoing sigma(4)-induced helix extension to trap DdvS. Altogether, we provide structural-mechanistic insights into membrane anti-sigma-ECF sigma regulation of an antiviral CBASS-CRISPR-Cas defense island. | ||
| - | + | Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-sigma factor and its ECF sigma partner.,Bernal-Bernal D, Pantoja-Uceda D, Lopez-Alonso JP, Lopez-Rojo A, Lopez-Ruiz JA, Galbis-Martinez M, Ochoa-Lizarralde B, Tascon I, Elias-Arnanz M, Ubarretxena-Belandia I, Padmanabhan S Sci Adv. 2024 Oct 25;10(43):eadp1053. doi: 10.1126/sciadv.adp1053. Epub 2024 Oct , 25. PMID:39454004<ref>PMID:39454004</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8rlz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Myxococcus xanthus]] | ||
| + | [[Category: Padmanabhan S]] | ||
| + | [[Category: Pantoja-Uceda D]] | ||
Current revision
NMR solution structure of the N-terminal cytoplasmic domain, DdvANt, of the membrane antisigma factor DdvA
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