8v62
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 1D10 and 4C06== | |
| + | <StructureSection load='8v62' size='340' side='right'caption='[[8v62]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8v62]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_respirovirus_3 Human respirovirus 3] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8V62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8V62 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v62 OCA], [https://pdbe.org/8v62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v62 RCSB], [https://www.ebi.ac.uk/pdbsum/8v62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v62 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A023PFZ0_9MONO A0A023PFZ0_9MONO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Respirovirus 3 is a leading cause of severe acute respiratory infections in vulnerable human populations. Entry into host cells is facilitated by the attachment glycoprotein and the fusion glycoprotein (F). Because of its crucial role, F represents an attractive therapeutic target. Here, we identify 13 F-directed heavy-chain-only antibody fragments that neutralize recombinant respirovirus 3. High-resolution cryo-EM structures of antibody fragments bound to the prefusion conformation of F reveal three distinct, previously uncharacterized epitopes. All three antibody fragments bind quaternary epitopes on F, suggesting mechanisms for neutralization that may include stabilization of the prefusion conformation. Studies in cotton rats demonstrate the prophylactic efficacy of these antibody fragments in reducing viral load in the lungs and nasal passages. These data highlight the potential of heavy-chain-only antibody fragments as effective interventions against respirovirus 3 infection and identify neutralizing epitopes that can be targeted for therapeutic development. | ||
| - | + | Structural basis for potent neutralization of human respirovirus type 3 by protective single-domain camelid antibodies.,Johnson NV, van Scherpenzeel RC, Bakkers MJG, Ramamohan AR, van Overveld D, Le L, Langedijk JPM, Kolkman JA, McLellan JS Nat Commun. 2024 Jun 27;15(1):5458. doi: 10.1038/s41467-024-49757-1. PMID:38937429<ref>PMID:38937429</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Johnson | + | <div class="pdbe-citations 8v62" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Ramamohan | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human respirovirus 3]] | ||
| + | [[Category: Lama glama]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Johnson NJ]] | ||
| + | [[Category: McLellan JS]] | ||
| + | [[Category: Ramamohan AR]] | ||
Current revision
Structure of the Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 1D10 and 4C06
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