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1rcb

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(New page: 200px<br /> <applet load="1rcb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rcb, resolution 2.25&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1rcb.gif|left|200px]]<br />
 
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<applet load="1rcb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rcb, resolution 2.25&Aring;" />
 
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'''CRYSTAL STRUCTURE OF HUMAN RECOMBINANT INTERLEUKIN-4 AT 2.25 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF HUMAN RECOMBINANT INTERLEUKIN-4 AT 2.25 ANGSTROMS RESOLUTION==
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The crystal structure of human recombinant interleukin-4 (IL-4) has been, solved by multiple isomorphous replacement, and refined to an R factor of, 0.218 at 2.25 A resolution. The molecule is a left-handed four-helix, bundle with a short stretch of beta sheet. The structure bears close, resemblance to other cytokines such as granulocyte-macrophage colony, stimulating factor (GM-CSF). Although no sequence similarity of IL-4 to, GM-CSF and other related cytokines has been previously postulated, structure-based alignment of IL-4 and GM-CSF revealed that the core of the, molecules, including large parts of all four helices and extending over, half of the molecule, has 30% sequence identity. This may have identified, regions which are not only important to maintain structure, but could also, play a role in receptor binding.
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<StructureSection load='1rcb' size='340' side='right'caption='[[1rcb]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rcb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcb OCA], [https://pdbe.org/1rcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcb RCSB], [https://www.ebi.ac.uk/pdbsum/1rcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcb ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/IL4_HUMAN IL4_HUMAN] Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:[https://omim.org/entry/601367 601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.<ref>PMID:14681304</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/IL4_HUMAN IL4_HUMAN] Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rcb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rcb ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: AIDS, slow progression to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147781 147781]], Atopy, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147781 147781]]
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*[[Interleukin 3D structures|Interleukin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1RCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RCB OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of human recombinant interleukin-4 at 2.25 A resolution., Wlodawer A, Pavlovsky A, Gustchina A, FEBS Lett. 1992 Aug 31;309(1):59-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1511746 1511746]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gustchina, A.]]
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[[Category: Gustchina A]]
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[[Category: Pavlovsky, A.]]
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[[Category: Pavlovsky A]]
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[[Category: Wlodaver, A.]]
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[[Category: Wlodawer A]]
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[[Category: cytokine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:02:06 2007''
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CRYSTAL STRUCTURE OF HUMAN RECOMBINANT INTERLEUKIN-4 AT 2.25 ANGSTROMS RESOLUTION

PDB ID 1rcb

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