1pmo

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[[Image:1pmo.gif|left|200px]]
 
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==Crystal structure of Escherichia coli GadB (neutral pH)==
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The line below this paragraph, containing "STRUCTURE_1pmo", creates the "Structure Box" on the page.
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<StructureSection load='1pmo' size='340' side='right'caption='[[1pmo]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pmo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PMO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLR:(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>PLR</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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{{STRUCTURE_1pmo| PDB=1pmo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmo OCA], [https://pdbe.org/1pmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pmo RCSB], [https://www.ebi.ac.uk/pdbsum/1pmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pmo ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of Escherichia coli GadB (neutral pH)'''
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== Function ==
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[https://www.uniprot.org/uniprot/DCEB_ECOLI DCEB_ECOLI]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Glutamate decarboxylase is a vitamin B6-dependent enzyme, which catalyses the decarboxylation of glutamate to gamma-aminobutyrate. In Escherichia coli, expression of glutamate decarboxylase (GadB), a 330 kDa hexamer, is induced to maintain the physiological pH under acidic conditions, like those of the passage through the stomach en route to the intestine. GadB, together with the antiporter GadC, constitutes the gad acid resistance system, which confers the ability for bacterial survival for at least 2 h in a strongly acidic environment. GadB undergoes a pH-dependent conformational change and exhibits an activity optimum at low pH. We determined the crystal structures of GadB at acidic and neutral pH. They reveal the molecular details of the conformational change and the structural basis for the acidic pH optimum. We demonstrate that the enzyme is localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls. We show by structure-based site-directed mutagenesis that the triple helix bundle formed by the N-termini of the protein at acidic pH is the major determinant for this behaviour.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pm/1pmo_consurf.spt"</scriptWhenChecked>
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1PMO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMO OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase., Capitani G, De Biase D, Aurizi C, Gut H, Bossa F, Grutter MG, EMBO J. 2003 Aug 15;22(16):4027-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12912902 12912902]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pmo ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Glutamate decarboxylase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Aurizi C]]
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[[Category: Aurizi, C.]]
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[[Category: Bossa F]]
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[[Category: Biase, D De.]]
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[[Category: Capitani G]]
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[[Category: Bossa, F.]]
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[[Category: De Biase D]]
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[[Category: Capitani, G.]]
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[[Category: Grutter MG]]
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[[Category: Grutter, M G.]]
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[[Category: Gut H]]
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[[Category: Gut, H.]]
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[[Category: Neutral-ph form of gadb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:15:19 2008''
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Current revision

Crystal structure of Escherichia coli GadB (neutral pH)

PDB ID 1pmo

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