1pn8

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[[Image:1pn8.gif|left|200px]]
 
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==Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.==
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The line below this paragraph, containing "STRUCTURE_1pn8", creates the "Structure Box" on the page.
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<SX load='1pn8' size='340' side='right' viewer='molstar' caption='[[1pn8]], [[Resolution|resolution]] 10.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pn8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PN8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pn8 OCA], [https://pdbe.org/1pn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pn8 RCSB], [https://www.ebi.ac.uk/pdbsum/1pn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pn8 ProSAT]</span></td></tr>
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{{STRUCTURE_1pn8| PDB=1pn8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS12_THET8 RS12_THET8] With S4 and S5 plays an important role in translational accuracy (By similarity).[HAMAP-Rule:MF_00403_B] Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.[HAMAP-Rule:MF_00403_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pn/1pn8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pn8 ConSurf].
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<div style="clear:both"></div>
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'''Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.'''
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==See Also==
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*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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==Overview==
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
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__TOC__
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</SX>
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==About this Structure==
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[[Category: Large Structures]]
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1PN8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PN8 OCA].
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==Reference==
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Locking and unlocking of ribosomal motions., Valle M, Zavialov A, Sengupta J, Rawat U, Ehrenberg M, Frank J, Cell. 2003 Jul 11;114(1):123-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12859903 12859903]
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[[Category: Protein complex]]
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Ehrenberg, M.]]
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[[Category: Ehrenberg M]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Rawat, U.]]
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[[Category: Rawat U]]
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[[Category: Sengupta, J.]]
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[[Category: Sengupta J]]
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[[Category: Valle, M.]]
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[[Category: Valle M]]
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[[Category: Zavialov, A.]]
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[[Category: Zavialov A]]
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[[Category: Ribosomal protein]]
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[[Category: Trna]]
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[[Category: Trna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:16:23 2008''
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Current revision

Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.

1pn8, resolution 10.80Å

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OCA

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