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1pnb

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[[Image:1pnb.gif|left|200px]]
 
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==STRUCTURE OF NAPIN BNIB, NMR, 10 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_1pnb", creates the "Structure Box" on the page.
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<StructureSection load='1pnb' size='340' side='right'caption='[[1pnb]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pnb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_napus Brassica napus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PNB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnb OCA], [https://pdbe.org/1pnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pnb RCSB], [https://www.ebi.ac.uk/pdbsum/1pnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnb ProSAT]</span></td></tr>
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{{STRUCTURE_1pnb| PDB=1pnb | SCENE= }}
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</table>
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== Function ==
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'''STRUCTURE OF NAPIN BNIB, NMR, 10 STRUCTURES'''
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[https://www.uniprot.org/uniprot/2SSI_BRANA 2SSI_BRANA] The small, basic, water-soluble napins are one of the two major kinds of storage proteins synthesized in the seed during its maturation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Napin BnIb is a representative member of the 2S albumin seed proteins, which consists of two polypeptide chains of 3.8 and 8.4 kDa linked by two disulfide bridges. In this work, a complete assignment of the 1H spectra of napin BnIb has been carried out by two-dimensional NMR sequence-specific methods and its secondary structure determined on the basis of spectral data. A calculation of the tertiary structure has been performed using approximately 500 distance constraints derived from unambiguously assigned NOE cross-correlations and distance geometry methods. The resulting global fold consists of five helices and a C-terminal loop arranged in a right-handed spiral. The folded protein is stabilized by two interchain disulfide bridges and two additional ones between cysteine residues in the large chain. The structure of napin BnIb represents a third example of a new and distinctive folding pattern first described for the hydrophobic protein from soybean and nonspecific lipid transfer proteins from wheat and maize. The presence of an internal cavity is not at all evident, which rules out in principle the napin BnIb as a carrier of lipids. The determined structure is compatible with activities attributed to these proteins such as phospholipid vesicle interaction, allergenicity, and calmodulin antagonism. Given the sequence homology of BnIb with other napins and napin-type 2S albumin seed proteins from different species, it is likely that all these proteins share a common architecture. The determined structure will be crucial to establish structure-function relationships and to explore the mechanisms of folding, processing, and deposition of these proteins. It will also provide a firm basis for a rational use of genetic engineering in order to develop improved transgenic plants.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pn/1pnb_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1PNB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_napus Brassica napus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNB OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pnb ConSurf].
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1H NMR assignment and global fold of napin BnIb, a representative 2S albumin seed protein., Rico M, Bruix M, Gonzalez C, Monsalve RI, Rodriguez R, Biochemistry. 1996 Dec 10;35(49):15672-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8961930 8961930]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Brassica napus]]
[[Category: Brassica napus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Bruix, M.]]
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[[Category: Bruix M]]
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[[Category: Gonzalez, C.]]
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[[Category: Gonzalez C]]
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[[Category: Monsalve, R.]]
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[[Category: Monsalve R]]
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[[Category: Rico, M.]]
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[[Category: Rico M]]
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[[Category: Rodriguez, R.]]
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[[Category: Rodriguez R]]
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[[Category: Albumin seed protein]]
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[[Category: Napin ia and ib]]
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[[Category: Seed storage protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:16:32 2008''
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Current revision

STRUCTURE OF NAPIN BNIB, NMR, 10 STRUCTURES

PDB ID 1pnb

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