1rh2

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(New page: 200px<br /> <applet load="1rh2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rh2, resolution 2.9&Aring;" /> '''RECOMBINANT HUMAN IN...)
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[[Image:1rh2.gif|left|200px]]<br />
 
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<applet load="1rh2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rh2, resolution 2.9&Aring;" />
 
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'''RECOMBINANT HUMAN INTERFERON-ALPHA 2B'''<br />
 
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==Overview==
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==RECOMBINANT HUMAN INTERFERON-ALPHA 2B==
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BACKGROUND: The human alpha-interferon (huIFN-alpha) family displays broad, spectrum antiviral, antiproliferative and immunomodulatory activities on a, variety of cell types. The diverse biological activities of the, IFN-alpha's are conveyed to cells through specific interactions with, cell-surface receptors. Despite considerable effort, no crystal structure, of a member of this family has yet been reported, because the quality of, the protein crystals have been unsuitable for crystallographic studies., Until now, structural models of the IFN-alpha's have been based on the, structure of murine IFN-beta (muIFN-beta). These models are likely to be, inaccurate, as the amino acid sequence of muIFN-beta differs significantly, from the IFN-alpha's at proposed receptor-binding sites. Structural, information on a huIFN-alpha subtype would provide an improved basis for, modeling the structures of the entire IFN-alpha family. RESULTS: The, crystal structure of recombinant human interferon-alpha 2b (huIFN-alpha, 2b) has been determined at 2.9 A resolution. HuIFN-alpha 2b exists in the, crystal as a noncovalent dimer, which associates in a novel manner. Unlike, other structurally characterized cytokines, extensive interactions in the, dimer interface are mediated by a zinc ion (Zn2+). The overall fold of, huIFN-alpha 2b is most similar to the structure of muIFN-beta. Unique to, huIFN-alpha 2b is a 3(10) helix in the AB loop which is held to the core, of the molecule by a disulfide bond. CONCLUSIONS: The structure of, huIFN-alpha 2b provides an accurate model for analysis of the &gt; 15 related, type 1 interferon molecules. HuIFN-alpha 2b displays considerable, structural similarity with muIFN-beta, interleukin-10 and, interferon-gamma, which also bind related class 2 cytokine receptors. From, these structural comparisons and numerous studies on the effects of, mutations on biological activity, we have identified protein surfaces that, appear to be important in receptor activation. This study also reveals the, potential biological importance of the huIFN-alpha 2b dimer.
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<StructureSection load='1rh2' size='340' side='right'caption='[[1rh2]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rh2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh2 OCA], [https://pdbe.org/1rh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh2 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IFNA2_HUMAN IFNA2_HUMAN] Produced by macrophages, IFN-alpha have antiviral activities.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/1rh2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rh2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RH2 OCA].
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*[[Interferon 3D structures|Interferon 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Zinc mediated dimer of human interferon-alpha 2b revealed by X-ray crystallography., Radhakrishnan R, Walter LJ, Hruza A, Reichert P, Trotta PP, Nagabhushan TL, Walter MR, Structure. 1996 Dec 15;4(12):1453-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8994971 8994971]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Walter, M.R.]]
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[[Category: Walter MR]]
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[[Category: ZN]]
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[[Category: 4 helix bundle]]
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[[Category: anti-viral]]
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[[Category: cytokine]]
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[[Category: immunomodulator]]
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[[Category: interferon]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:03:38 2007''
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Current revision

RECOMBINANT HUMAN INTERFERON-ALPHA 2B

PDB ID 1rh2

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