1rho

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(New page: 200px<br /> <applet load="1rho" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rho, resolution 2.50&Aring;" /> '''STRUCTURE OF RHO GU...)
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[[Image:1rho.gif|left|200px]]<br />
 
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<applet load="1rho" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rho, resolution 2.50&Aring;" />
 
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'''STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR'''<br />
 
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==Overview==
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==STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR==
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BACKGROUND: The rho family of small G proteins, including rho, rac and, cdc42, are involved in many cellular processes, including cell, transformation by ras and the organization of the actin cytoskeleton., Additionally, rac has a role in the regulation of phagocyte NADPH oxidase., Guanine nucleotide dissociation inhibitors (GDIs) of the rhoGDI family, bind to these G proteins and regulate their activity by preventing, nucleotide dissociation and by controlling their interaction with, membranes. RESULTS: We report the structure of rhoGDI, determined by a, combination of X-ray crystallography and NMR spectroscopy. NMR, spectroscopy and selective proteolysis show that the N-terminal 50-60, residues of rhoGDI are flexible and unstructured in solution. The 2.5 A, crystal structure of the folded core of rhoGDI, comprising residues, 59-204, shows it to have an immunoglobulin-like fold, with an, unprecedented insertion of two short beta strands and a 310 helix. There, is an unusual pocket between the beta sheets of the immunoglobulin fold, which may bind the C-terminal isoprenyl group of rac. NMR spectroscopy, shows that the N-terminal arm is necessary for binding rac, although it, remains largely flexible even in the complex. CONCLUSIONS: The rhoGDI, structure is notable for the existence of both a structured and a highly, flexible domain, both of which appear to be required for the interaction, with rac. The immunoglobulin-like fold of the structured domain is unusual, for a cytoplasmic protein. The presence of equivalent cleavage sites in, rhoGDI and the closely related D4/Ly-GDI (rhoGDI-2) suggest that, proteolytic cleavage between the flexible and structured regions of rhoGDI, may have a role in the regulation of the activity of members of this, family. There is no detectable similarity between the structure of rhoGDI, and the recently reported structure of rabGDI, which performs the same, function as rhoGDI for the rab family of small G proteins.
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<StructureSection load='1rho' size='340' side='right'caption='[[1rho]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rho]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RHO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rho OCA], [https://pdbe.org/1rho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rho RCSB], [https://www.ebi.ac.uk/pdbsum/1rho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rho ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GDIR1_HUMAN GDIR1_HUMAN] Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1 (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/1rho_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rho ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RHO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RHO OCA].
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*[[Guanine nucleotide dissociation inhibitor|Guanine nucleotide dissociation inhibitor]]
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__TOC__
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==Reference==
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</StructureSection>
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A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm., Keep NH, Barnes M, Barsukov I, Badii R, Lian LY, Segal AW, Moody PC, Roberts GC, Structure. 1997 May 15;5(5):623-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9195882 9195882]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Keep, N.H.]]
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[[Category: Keep NH]]
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[[Category: Moody, P.C.E.]]
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[[Category: Moody PCE]]
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[[Category: Roberts, G.C.K.]]
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[[Category: Roberts GCK]]
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[[Category: SO4]]
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[[Category: gtpase activation]]
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[[Category: inhibitor]]
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[[Category: phosphorylation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:03:56 2007''
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Current revision

STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR

PDB ID 1rho

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