1pry

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[[Image:1pry.jpg|left|200px]]
 
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==Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)==
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The line below this paragraph, containing "STRUCTURE_1pry", creates the "Structure Box" on the page.
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<StructureSection load='1pry' size='340' side='right'caption='[[1pry]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pry]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pry OCA], [https://pdbe.org/1pry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pry RCSB], [https://www.ebi.ac.uk/pdbsum/1pry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pry ProSAT], [https://www.topsan.org/Proteins/SECSG/1pry TOPSAN]</span></td></tr>
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{{STRUCTURE_1pry| PDB=1pry | SCENE= }}
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</table>
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== Function ==
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'''Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)'''
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[https://www.uniprot.org/uniprot/FLPA_PYRFU FLPA_PYRFU] Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity).[HAMAP-Rule:MF_00351]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The methyltransferase fibrillarin is the catalytic component of ribonucleoprotein complexes that direct site-specific methylation of precursor ribosomal RNA and are critical for ribosome biogenesis in eukaryotes and archaea. Here we report the crystal structure of a fibrillarin ortholog from the hyperthermophilic archaeon Pyrococcus furiosus at 1.97A resolution. Comparisons of the X-ray structures of fibrillarin orthologs from Methanococcus jannashii and Archaeoglobus fulgidus reveal nearly identical backbone configurations for the catalytic C-terminal domain with the exception of a unique loop conformation at the S-adenosyl-l-methionine (AdoMet) binding pocket in P. furiosus. In contrast, the N-terminal domains are divergent which may explain why some forms of fibrillarin apparently homodimerize (M. jannashii) while others are monomeric (P. furiosus and A. fulgidus). Three positively charged amino acids surround the AdoMet-binding site and sequence analysis indicates that this is a conserved feature of both eukaryotic and archaeal fibrillarins. We discuss the possibility that these basic residues of fibrillarin are important for RNA-guided rRNA methylation.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1pry_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1PRY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRY OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pry ConSurf].
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Structure determination of fibrillarin from the hyperthermophilic archaeon Pyrococcus furiosus., Deng L, Starostina NG, Liu ZJ, Rose JP, Terns RM, Terns MP, Wang BC, Biochem Biophys Res Commun. 2004 Mar 12;315(3):726-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14975761 14975761]
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<div style="clear:both"></div>
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[[Category: Pyrococcus furiosus]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Deng, L.]]
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[[Category: Large Structures]]
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[[Category: Liu, Z J.]]
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[[Category: Rose, J P.]]
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[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
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[[Category: Starostina, N G.]]
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[[Category: Terns, M P.]]
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[[Category: Terns, R M.]]
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[[Category: Wang, B C.]]
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[[Category: Fibrillarin]]
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[[Category: Methylation]]
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[[Category: Pfu-65527]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Ribosomal rna processing]]
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[[Category: Deng L]]
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[[Category: Secsg]]
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[[Category: Liu Z-J]]
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[[Category: Snornp]]
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[[Category: Rose JP]]
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[[Category: Southeast collaboratory for structural genomic]]
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[[Category: Starostina NG]]
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[[Category: Structural genomic]]
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[[Category: Terns MP]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:25:03 2008''
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[[Category: Terns RM]]
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[[Category: Wang B-C]]

Current revision

Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)

PDB ID 1pry

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