1pt3

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[[Image:1pt3.gif|left|200px]]
 
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==Crystal structures of nuclease-ColE7 complexed with octamer DNA==
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The line below this paragraph, containing "STRUCTURE_1pt3", creates the "Structure Box" on the page.
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<StructureSection load='1pt3' size='340' side='right'caption='[[1pt3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1pt3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PT3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pt3 OCA], [https://pdbe.org/1pt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pt3 RCSB], [https://www.ebi.ac.uk/pdbsum/1pt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pt3 ProSAT]</span></td></tr>
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{{STRUCTURE_1pt3| PDB=1pt3 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CEA7_ECOLX CEA7_ECOLX] This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/1pt3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pt3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The bacterial toxin ColE7 bears an HNH motif which has been identified in hundreds of prokaryotic and eukaryotic endonucleases, involved in DNA homing, restriction, repair, or chromosome degradation. The crystal structure of the nuclease domain of ColE7 in complex with a duplex DNA has been determined at 2.5 A resolution. The HNH motif is bound at the minor groove primarily to DNA phosphate groups at and beyond the 3' side of the scissile phosphate, with little interaction with ribose groups and bases. This result provides a structural basis for sugar- and sequence-independent DNA recognition and the inhibition mechanism by inhibitor Im7, which blocks the substrate binding site but not the active site. Structural comparison shows that two families of endonucleases bind and bend DNA in a similar way to that of the HNH ColE7, indicating that endonucleases containing a "betabetaalpha-metal" fold of active site possess a universal mode for protein-DNA interactions.
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'''Crystal structures of nuclease-ColE7 complexed with octamer DNA'''
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DNA binding and degradation by the HNH protein ColE7.,Hsia KC, Chak KF, Liang PH, Cheng YS, Ku WY, Yuan HS Structure. 2004 Feb;12(2):205-14. PMID:14962381<ref>PMID:14962381</ref>
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==Overview==
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The bacterial toxin ColE7 bears an HNH motif which has been identified in hundreds of prokaryotic and eukaryotic endonucleases, involved in DNA homing, restriction, repair, or chromosome degradation. The crystal structure of the nuclease domain of ColE7 in complex with a duplex DNA has been determined at 2.5 A resolution. The HNH motif is bound at the minor groove primarily to DNA phosphate groups at and beyond the 3' side of the scissile phosphate, with little interaction with ribose groups and bases. This result provides a structural basis for sugar- and sequence-independent DNA recognition and the inhibition mechanism by inhibitor Im7, which blocks the substrate binding site but not the active site. Structural comparison shows that two families of endonucleases bind and bend DNA in a similar way to that of the HNH ColE7, indicating that endonucleases containing a "betabetaalpha-metal" fold of active site possess a universal mode for protein-DNA interactions.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1PT3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PT3 OCA].
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</div>
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<div class="pdbe-citations 1pt3" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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DNA binding and degradation by the HNH protein ColE7., Hsia KC, Chak KF, Liang PH, Cheng YS, Ku WY, Yuan HS, Structure. 2004 Feb;12(2):205-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962381 14962381]
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*[[Colicin 3D structures|Colicin 3D structures]]
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Chak, K F.]]
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__TOC__
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[[Category: Cheng, Y S.]]
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</StructureSection>
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[[Category: Hsia, K C.]]
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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[[Category: Ku, W Y.]]
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[[Category: Large Structures]]
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[[Category: Yuan, H S.]]
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[[Category: Chak KF]]
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[[Category: Colicin]]
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[[Category: Cheng YS]]
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[[Category: Endonuclease]]
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[[Category: Hsia KC]]
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[[Category: Hnh motif]]
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[[Category: Ku WY]]
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[[Category: Protein-dna complex]]
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[[Category: Yuan HS]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:27:25 2008''
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Current revision

Crystal structures of nuclease-ColE7 complexed with octamer DNA

PDB ID 1pt3

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