8rtf
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8rtf is ON HOLD Authors: Sterckx, Y.G.-J. Description: Crystal structure of Trypanosoma congolense pyruvate kinase in complex with a single-domain ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Trypanosoma congolense pyruvate kinase in complex with a single-domain antibody (TcoPYK-sdAb42)== | |
| + | <StructureSection load='8rtf' size='340' side='right'caption='[[8rtf]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8rtf]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_congolense Trypanosoma congolense] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RTF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rtf OCA], [https://pdbe.org/8rtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rtf RCSB], [https://www.ebi.ac.uk/pdbsum/8rtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rtf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G0UYF4_TRYCI G0UYF4_TRYCI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | African trypanosomes are the causative agents of neglected tropical diseases affecting both humans and livestock. Disease control is highly challenging due to an increasing number of drug treatment failures. African trypanosomes are extracellular, blood-borne parasites that mainly rely on glycolysis for their energy metabolism within the mammalian host. Trypanosomal glycolytic enzymes are therefore of interest for the development of trypanocidal drugs. Here, we report the serendipitous discovery of a camelid single-domain antibody (sdAb aka Nanobody) that selectively inhibits the enzymatic activity of trypanosomatid (but not host) pyruvate kinases through an allosteric mechanism. By combining enzyme kinetics, biophysics, structural biology, and transgenic parasite survival assays, we provide a proof-of-principle that the sdAb-mediated enzyme inhibition negatively impacts parasite fitness and growth. | ||
| - | + | Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody.,Pinto Torres JE, Claes M, Hendrickx R, Yuan M, Smiejkowska N, Van Wielendaele P, Hacisuleyman A, De Winter H, Muyldermans S, Michels PAM, Walkinshaw MD, Versees W, Caljon G, Magez S, Sterckx YG Elife. 2025 Mar 31;13:RP100066. doi: 10.7554/eLife.100066. PMID:40163365<ref>PMID:40163365</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Sterckx | + | <div class="pdbe-citations 8rtf" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Trypanosoma congolense]] | ||
| + | [[Category: Vicugna pacos]] | ||
| + | [[Category: Sterckx YG-J]] | ||
Current revision
Crystal structure of Trypanosoma congolense pyruvate kinase in complex with a single-domain antibody (TcoPYK-sdAb42)
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