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1px8

From Proteopedia

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[[Image:1px8.gif|left|200px]]
 
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==Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase==
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The line below this paragraph, containing "STRUCTURE_1px8", creates the "Structure Box" on the page.
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<StructureSection load='1px8' size='340' side='right'caption='[[1px8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1px8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_saccharolyticum Thermoanaerobacterium saccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PX8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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{{STRUCTURE_1px8| PDB=1px8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1px8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1px8 OCA], [https://pdbe.org/1px8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1px8 RCSB], [https://www.ebi.ac.uk/pdbsum/1px8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1px8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XYNB_THESA XYNB_THESA] Has hydrolytic activity towards xylopentaose, xylotriose, xylobiose and P-nitrophenyl-beta-D-xylopyranoside, but has no activity toward xylan.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/px/1px8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1px8 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase'''
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==See Also==
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*[[Xylosidase 3D structures|Xylosidase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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1,4-beta-D-Xylan is the major component of plant cell-wall hemicelluloses. beta-D-Xylosidases are involved in the breakdown of xylans into xylose and belong to families 3, 39, 43, 52, and 54 of glycoside hydrolases. Here, we report the first crystal structure of a member of family 39 glycoside hydrolase, i.e. beta-D-xylosidase from Thermoanaerobacterium saccharolyticum strain B6A-RI. This study also represents the first structure of any beta-xylosidase of the above five glycoside hydrolase families. Each monomer of T. saccharolyticum beta-xylosidase comprises three distinct domains; a catalytic domain of the canonical (beta/alpha)(8)-barrel fold, a beta-sandwich domain, and a small alpha-helical domain. We have determined the structure in two forms: D-xylose-bound enzyme and a covalent 2-deoxy-2-fluoro-alpha-D-xylosyl-enzyme intermediate complex, thus providing two snapshots in the reaction pathway. This study provides structural evidence for the proposed double displacement mechanism that involves a covalent intermediate. Furthermore, it reveals possible functional roles for His228 as the auxiliary acid/base and Glu323 as a key residue in substrate recognition.
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[[Category: Large Structures]]
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==About this Structure==
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1PX8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_saccharolyticum Thermoanaerobacterium saccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PX8 OCA].
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==Reference==
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Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase., Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW, J Mol Biol. 2004 Jan 2;335(1):155-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14659747 14659747]
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[[Category: Single protein]]
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[[Category: Thermoanaerobacterium saccharolyticum]]
[[Category: Thermoanaerobacterium saccharolyticum]]
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[[Category: Xylan 1,4-beta-xylosidase]]
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[[Category: Ahn HJ]]
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[[Category: Ahn, H J.]]
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[[Category: Berendzen J]]
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[[Category: Berendzen, J.]]
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[[Category: Il Lee B]]
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[[Category: Laivenieks, M.]]
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[[Category: Laivenieks M]]
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[[Category: Lee, B Il.]]
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[[Category: Liong EC]]
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[[Category: Liong, E C.]]
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[[Category: Pedelacq JD]]
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[[Category: Pedelacq, J D.]]
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[[Category: Suh SW]]
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[[Category: Suh, S W.]]
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[[Category: Vieille C]]
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[[Category: Vieille, C.]]
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[[Category: Vocadlo DJ]]
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[[Category: Vocadlo, D J.]]
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[[Category: Withers SG]]
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[[Category: Withers, S G.]]
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[[Category: Yang JK]]
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[[Category: Yang, J K.]]
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[[Category: Yoon HJ]]
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[[Category: Yoon, H J.]]
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[[Category: Zeikus GJ]]
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[[Category: Zeikus, G J.]]
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[[Category: Covalent glycosyl-enzyme intermediate]]
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[[Category: Family 39 glycoside hydrolase]]
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[[Category: Xylan]]
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[[Category: Xylose]]
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[[Category: Xylosidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:35:34 2008''
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Current revision

Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase

PDB ID 1px8

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