1rrp

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(New page: 200px<br /> <applet load="1rrp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rrp, resolution 2.96&Aring;" /> '''STRUCTURE OF THE RA...)
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[[Image:1rrp.gif|left|200px]]<br />
 
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<applet load="1rrp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rrp, resolution 2.96&Aring;" />
 
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'''STRUCTURE OF THE RAN-GPPNHP-RANBD1 COMPLEX'''<br />
 
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==Overview==
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==STRUCTURE OF THE RAN-GPPNHP-RANBD1 COMPLEX==
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The protein Ran is a small GTP-binding protein that binds to two types of, effector inside the cell: Ran-binding proteins, which have a role in, terminating export processes from the nucleus to the cytoplasm, and, importin-beta-like molecules that bind cargo proteins during nuclear, transport. The Ran-binding domain is a conserved sequence motif found in, several proteins that participate in these transport processes. The, Ran-binding protein RanBP2 contains four of these domains and constitutes, a large part of the cytoplasmic fibrils that extend from the nuclear-pore, complex. The structure of Ran bound to a non-hydrolysable GTP analogue, (Ran x GppNHp) in complex with the first Ran-binding domain (RanBD1) of, human RanBP2 reveals not only that RanBD1 has a pleckstrin-homology domain, fold, but also that the switch-I region of Ran x GppNHp resembles the, canonical Ras GppNHp structure and that the carboxy terminus of Ran is, wrapped around RanBD1, contacting a basic patch on RanBD1 through its, acidic end. This molecular 'embrace' enables RanBDs to sequester the Ran, carboxy terminus, triggering the dissociation of Ran x GTP from, importin-beta-related transport factors and facilitating GTP hydrolysis by, the GTPase-activating protein ranGAP. Such a mechanism represents a new, type of switch mechanism and regulatory protein-protein interaction for a, Ras-related protein.
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<StructureSection load='1rrp' size='340' side='right'caption='[[1rrp]], [[Resolution|resolution]] 2.96&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rrp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RRP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.96&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rrp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rrp OCA], [https://pdbe.org/1rrp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rrp RCSB], [https://www.ebi.ac.uk/pdbsum/1rrp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rrp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rr/1rrp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rrp ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: Osteolysis, familial expansile OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=603499 603499]], Paget disease of bone OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=603499 603499]]
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1RRP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RRP OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport., Vetter IR, Nowak C, Nishimoto T, Kuhlmann J, Wittinghofer A, Nature. 1999 Mar 4;398(6722):39-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10078529 10078529]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Kuhlmann, J.]]
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[[Category: Kuhlmann J]]
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[[Category: Nishimoto, T.]]
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[[Category: Nishimoto T]]
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[[Category: Nowak, C.]]
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[[Category: Nowak C]]
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[[Category: Vetter, I.R.]]
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[[Category: Vetter IR]]
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[[Category: Wittinghofer, A.]]
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[[Category: Wittinghofer A]]
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[[Category: GNP]]
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[[Category: MG]]
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[[Category: complex (small gtpase/nuclear protein)]]
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[[Category: nuclear transport]]
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[[Category: small gtpase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:06:46 2007''
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Current revision

STRUCTURE OF THE RAN-GPPNHP-RANBD1 COMPLEX

PDB ID 1rrp

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