This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1q5z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:13, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1q5z.jpg|left|200px]]
 
-
<!--
+
==Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)==
-
The line below this paragraph, containing "STRUCTURE_1q5z", creates the "Structure Box" on the page.
+
<StructureSection load='1q5z' size='340' side='right'caption='[[1q5z]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1q5z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q5Z FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5z OCA], [https://pdbe.org/1q5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q5z RCSB], [https://www.ebi.ac.uk/pdbsum/1q5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q5z ProSAT]</span></td></tr>
-
{{STRUCTURE_1q5z| PDB=1q5z | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)'''
+
[https://www.uniprot.org/uniprot/SIPA_SALTY SIPA_SALTY] Actin-binding protein that interferes with host cell actin cytoskeleton. It stimulates actin polymerization and counteracts F-actin destabilizing proteins. Potentiates SipC activity; both are required for an efficient bacterial internalization. In vitro, forms a complex with host cell protein T-plastin increasing actin bundling. It inhibits ADF/cofilin-directed depolymerization both by preventing binding of ADF and cofilin and by displacing them from F-actin. Also protects F-actin from gelsolin-directed severing and reanneals gelsolin-severed F-actin fragments.<ref>PMID:10092234</ref> <ref>PMID:14992720</ref>
-
 
+
== References ==
-
 
+
<references/>
-
==Overview==
+
__TOC__
-
Like many bacterial pathogens, Salmonella spp. use a type III secretion system to inject virulence proteins into host cells. The Salmonella invasion protein A (SipA) binds host actin, enhances its polymerization near adherent extracellular bacteria, and contributes to cytoskeletal rearrangements that internalize the pathogen. By combining x-ray crystallography of SipA with electron microscopy and image analysis of SipA-actin filaments, we show that SipA functions as a "molecular staple," in which a globular domain and two nonglobular "arms" mechanically stabilize the filament by tethering actin subunits in opposing strands. Deletion analysis of the tethering arms provides strong support for this model.
+
</StructureSection>
-
 
+
[[Category: Large Structures]]
-
==About this Structure==
+
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-
1Q5Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].
+
[[Category: Egelman EH]]
-
 
+
[[Category: Galkin VE]]
-
==Reference==
+
[[Category: Lilic M]]
-
Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms., Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE, Science. 2003 Sep 26;301(5641):1918-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14512630 14512630]
+
[[Category: Orlova A]]
-
[[Category: Salmonella typhimurium]]
+
[[Category: Stebbins CE]]
-
[[Category: Single protein]]
+
[[Category: VanLoock MS]]
-
[[Category: Egelman, E H.]]
+
-
[[Category: Galkin, V E.]]
+
-
[[Category: Lilic, M.]]
+
-
[[Category: Orlova, A.]]
+
-
[[Category: Stebbins, C E.]]
+
-
[[Category: VanLoock, M S.]]
+
-
[[Category: Cell invasion]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:54:26 2008''
+

Current revision

Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)

PDB ID 1q5z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1q5z"
Personal tools