1q80

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[[Image:1q80.gif|left|200px]]
 
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==Solution structure and dynamics of Nereis sarcoplasmic calcium binding protein==
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The line below this paragraph, containing "STRUCTURE_1q80", creates the "Structure Box" on the page.
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<StructureSection load='1q80' size='340' side='right'caption='[[1q80]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1q80]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hediste_diversicolor Hediste diversicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q80 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q80 OCA], [https://pdbe.org/1q80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q80 RCSB], [https://www.ebi.ac.uk/pdbsum/1q80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q80 ProSAT]</span></td></tr>
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{{STRUCTURE_1q80| PDB=1q80 | SCENE= }}
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</table>
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== Function ==
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'''Solution structure and dynamics of Nereis sarcoplasmic calcium binding protein'''
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[https://www.uniprot.org/uniprot/SCP_HEDDI SCP_HEDDI] Like parvalbumins, SCP's seem to be more abundant in fast contracting muscles, but no functional relationship can be established from this distribution.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The solution structure of Nereis diversicolor sarcoplasmic calcium-binding protein (NSCP) in the calcium-bound form was determined by NMR spectroscopy, distance geometry and simulated annealing. Based on 1859 NOE restraints and 262 angular restraints, 17 structures were generated with a rmsd of 0.87 A from the mean structure. The solution structure, which is highly similar to the structure obtained by X-ray crystallography, includes two open EF-hand domains, which are in close contact through their hydrophobic surfaces. The internal dynamics of the protein backbone were determined by studying amide hydrogen/deuterium exchange rates and 15N nuclear relaxation. The two methods revealed a highly compact and rigid structure, with greatly restricted mobility at the two termini. For most of the amide protons, the free energy of exchange-compatible structural opening is similar to the free energy of structural stability, suggesting that isotope exchange of these protons takes place through global unfolding of the protein. Enhanced conformational flexibility was noted in the unoccupied Ca2+-binding site II, as well as the neighbouring helices. Analysis of the experimental nuclear relaxation and the molecular dynamics simulations give very similar profiles for the backbone generalized order parameter (S2), a parameter related to the amplitude of fast (picosecond to nanosecond) movements of N(H)-H vectors. We also noted a significant correlation between this parameter, the exchange rate, and the crystallographic B factor along the sequence.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q8/1q80_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1Q80 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neanthes_diversicolor Neanthes diversicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q80 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q80 ConSurf].
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Solution structure and internal dynamics of NSCP, a compact calcium-binding protein., Rabah G, Popescu R, Cox JA, Engelborghs Y, Craescu CT, FEBS J. 2005 Apr;272(8):2022-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15819893 15819893]
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<div style="clear:both"></div>
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[[Category: Neanthes diversicolor]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Cox, J A.]]
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[[Category: Hediste diversicolor]]
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[[Category: Craescu, C T.]]
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[[Category: Large Structures]]
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[[Category: Engelborghs, Y.]]
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[[Category: Cox JA]]
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[[Category: Popescu, R.]]
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[[Category: Craescu CT]]
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[[Category: Rabah, G.]]
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[[Category: Engelborghs Y]]
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[[Category: All-alpha]]
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[[Category: Popescu R]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:58:44 2008''
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[[Category: Rabah G]]

Current revision

Solution structure and dynamics of Nereis sarcoplasmic calcium binding protein

PDB ID 1q80

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