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7um0
From Proteopedia
(Difference between revisions)
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<StructureSection load='7um0' size='340' side='right'caption='[[7um0]], [[Resolution|resolution]] 3.80Å' scene=''> | <StructureSection load='7um0' size='340' side='right'caption='[[7um0]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[7um0]] is a | + | <table><tr><td colspan='2'>[[7um0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_AR9 Bacillus phage AR9] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UM0 FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | ||
| - | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7um0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7um0 OCA], [https://pdbe.org/7um0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7um0 RCSB], [https://www.ebi.ac.uk/pdbsum/7um0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7um0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7um0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7um0 OCA], [https://pdbe.org/7um0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7um0 RCSB], [https://www.ebi.ac.uk/pdbsum/7um0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7um0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A172JIC8_BPPB1 A0A172JIC8_BPPB1] | [https://www.uniprot.org/uniprot/A0A172JIC8_BPPB1 A0A172JIC8_BPPB1] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recognition of promoters in bacterial RNA polymerases (RNAPs) is controlled by sigma subunits. The key sequence motif recognized by the sigma, the -10 promoter element, is located in the non-template strand of the double-stranded DNA molecule ~10 nucleotides upstream of the transcription start site. Here, we explain the mechanism by which the phage AR9 non-virion RNAP (nvRNAP), a bacterial RNAP homolog, recognizes the -10 element of its deoxyuridine-containing promoter in the template strand. The AR9 sigma-like subunit, the nvRNAP enzyme core, and the template strand together form two nucleotide base-accepting pockets whose shapes dictate the requirement for the conserved deoxyuridines. A single amino acid substitution in the AR9 sigma-like subunit allows one of these pockets to accept a thymine thus expanding the promoter consensus. Our work demonstrates the extent to which viruses can evolve host-derived multisubunit enzymes to make transcription of their own genes independent of the host. | ||
| + | |||
| + | Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase.,Fraser A, Sokolova ML, Drobysheva AV, Gordeeva JV, Borukhov S, Jumper J, Severinov KV, Leiman PG Nat Commun. 2022 Jun 20;13(1):3526. doi: 10.1038/s41467-022-31214-6. PMID:35725571<ref>PMID:35725571</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7um0" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus phage AR9]] | [[Category: Bacillus phage AR9]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
[[Category: Fraser A]] | [[Category: Fraser A]] | ||
[[Category: Leiman PG]] | [[Category: Leiman PG]] | ||
[[Category: Sokolova ML]] | [[Category: Sokolova ML]] | ||
Current revision
Structure of the phage AR9 non-virion RNA polymerase holoenzyme in complex with two DNA oligonucleotides containing the AR9 P077 promoter as determined by cryo-EM
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