8w2o
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8w2o is ON HOLD Authors: Shi, S.S., Kuang, Z.L., Zhao, R. Description: Yeast U1 snRNP with humanized U1C Zinc-Finger domain [[Category: Unreleased ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Yeast U1 snRNP with humanized U1C Zinc-Finger domain== | |
| + | <StructureSection load='8w2o' size='340' side='right'caption='[[8w2o]], [[Resolution|resolution]] 3.49Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8w2o]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8W2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8W2O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.49Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8w2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8w2o OCA], [https://pdbe.org/8w2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8w2o RCSB], [https://www.ebi.ac.uk/pdbsum/8w2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8w2o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RU17_YEAST RU17_YEAST] Involved in nuclear mRNA splicing. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The recognition of 5' splice site (5' ss) is one of the earliest steps of pre-mRNA splicing. To better understand the mechanism and regulation of 5' ss recognition, we selectively humanized components of the yeast U1 snRNP to reveal the function of these components in 5' ss recognition and splicing. We targeted U1C and Luc7, two proteins that interact with and stabilize the yeast U1 (yU1) snRNA and the 5' ss RNA duplex. We replaced the Zinc-Finger (ZnF) domain of yU1C with its human counterpart, which resulted in a cold-sensitive growth phenotype and moderate splicing defects. We next added an auxin-inducible degron to yLuc7 protein (to mimic the lack of Luc7Ls in human U1 snRNP) and found that Luc7-depleted yU1 snRNP resulted in the concomitant loss of PRP40 and Snu71 (two other essential yeast U1 snRNP proteins), and further biochemical analyses suggest a model of how these three proteins interact with each other in the U1 snRNP. The loss of these proteins resulted in a significant growth retardation accompanied by a global suppression of pre-mRNA splicing. The splicing suppression led to mitochondrial dysfunction as revealed by a release of Fe2+ into the growth medium and an induction of mitochondrial reactive oxygen species. Together, these observations indicate that the human U1C ZnF can substitute that of yeast, Luc7 is essential for the incorporation of the Luc7-Prp40-Snu71 trimer into yeast U1 snRNP, and splicing plays a major role in the regulation of mitochondrial function in yeast. | ||
| - | + | , PMID:38688558<ref>PMID:38688558</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8w2o" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kuang | + | == References == |
| - | [[Category: Shi | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Kuang ZL]] | ||
| + | [[Category: Shi SS]] | ||
| + | [[Category: Zhao R]] | ||
Current revision
Yeast U1 snRNP with humanized U1C Zinc-Finger domain
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