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| - | [[Image:1ql2.gif|left|200px]] | |
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| - | <!--
| + | ==Inovirus (Filamentous Bacteriophage) Strain PF1 Major Coat Protein Assembly== |
| - | The line below this paragraph, containing "STRUCTURE_1ql2", creates the "Structure Box" on the page.
| + | <StructureSection load='1ql2' size='340' side='right'caption='[[1ql2]], [[Resolution|resolution]] 3.10Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1ql2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pf1 Pseudomonas virus Pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QL2 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Fiber diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
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| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ql2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ql2 OCA], [https://pdbe.org/1ql2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ql2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ql2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ql2 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1ql2| PDB=1ql2 | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''INOVIRUS (FILAMENTOUS BACTERIOPHAGE) STRAIN PF1 MAJOR COAT PROTEIN ASSEMBLY''' | + | [https://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). |
| - | | + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==Overview== | + | [[Category: Large Structures]] |
| - | The major coat protein in the capsid of Pf1 filamentous bacteriophage (Inovirus) forms a helical assembly of about 7000 identical protein subunits, each of which contains 46 amino-acid residues and can be closely approximated by a single gently curved alpha-helix. Since the viral DNA occupies the core of the tubular capsid and appears to make no significant specific interactions with the capsid proteins, the capsid is a simple model system for the study of the static and dynamic properties of alpha-helix assembly. The capsid undergoes a reversible temperature-induced structural transition at about 283 K between two slightly different helix forms. The two forms can coexist without an intermediate state, consistent with a first-order structural phase transition. The molecular model of the higher temperature form was refined using improved X-ray fibre diffraction data and new refinement and validation methods. The refinement indicates that the two forms are related by a change in the orientation of the capsid subunits within the virion, without a significant change in local conformation of the subunits. On the higher temperature diffraction pattern there is a region of observed intensity that is not consistent with a simple helix of identical subunits; it is proposed that the structure involves groups of three subunits which each have a slightly different orientation within the group. The grouping of subunits suggests that a change in subunit libration frequency could be the basis of the Pf1 structural transition; calculations from the model are used to explore this idea.
| + | [[Category: Pseudomonas virus Pf1]] |
| - | | + | [[Category: Marvin DA]] |
| - | ==About this Structure== | + | [[Category: Symmons MF]] |
| - | 1QL2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL2 OCA].
| + | [[Category: Welsh LC]] |
| - | | + | |
| - | ==Reference== | + | |
| - | The molecular structure and structural transition of the alpha-helical capsid in filamentous bacteriophage Pf1., Welsh LC, Symmons MF, Marvin DA, Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):137-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10666593 10666593]
| + | |
| - | [[Category: Pseudomonas phage pf1]] | + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Marvin, D A.]] | + | |
| - | [[Category: Symmons, M F.]]
| + | |
| - | [[Category: Welsh, L C.]] | + | |
| - | [[Category: Helical virus]] | + | |
| - | [[Category: Helical virus coat protein]] | + | |
| - | [[Category: Inovirus]] | + | |
| - | [[Category: Ssdna viruse]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:24:11 2008''
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