1srq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1srq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1srq, resolution 2.90&Aring;" /> '''Crystal Structure o...)
Current revision (08:33, 14 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1srq.gif|left|200px]]<br />
 
-
<applet load="1srq" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1srq, resolution 2.90&Aring;" />
 
-
'''Crystal Structure of the Rap1GAP catalytic domain'''<br />
 
-
==Overview==
+
==Crystal Structure of the Rap1GAP catalytic domain==
-
Rap1 is a Ras-like guanine-nucleotide-binding protein (GNBP) that is, involved in a variety of signal-transduction processes. It regulates, integrin-mediated cell adhesion and might activate extracellular, signal-regulated kinase. Like other Ras-like GNBPs, Rap1 is regulated by, guanine-nucleotide-exchange factors (GEFs) and GTPase-activating proteins, (GAPs). These GAPs increase the slow intrinsic GTPase reaction of Ras-like, GNBPs by many orders of magnitude and allow tight regulation of, signalling. The activation mechanism involves stabilization of the, catalytic glutamine of the GNBP and, in most cases, the insertion of a, catalytic arginine of GAP into the active site. Rap1 is a close homologue, of Ras but does not possess the catalytic glutamine essential for GTP, hydrolysis in all other Ras-like and Galpha proteins. Furthermore, RapGAPs, are not related to other GAPs and apparently do not use a catalytic, arginine residue. Here we present the crystal structure of the catalytic, domain of the Rap1-specific Rap1GAP at 2.9 A. By mutational analysis, fluorescence titration and stopped-flow kinetic assay, we demonstrate that, Rap1GAP provides a catalytic asparagine to stimulate GTP hydrolysis., Implications for the disease tuberous sclerosis are discussed.
+
<StructureSection load='1srq' size='340' side='right'caption='[[1srq]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1srq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SRQ FirstGlance]. <br>
-
1SRQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SRQ OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1srq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1srq OCA], [https://pdbe.org/1srq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1srq RCSB], [https://www.ebi.ac.uk/pdbsum/1srq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1srq ProSAT]</span></td></tr>
-
The GTPase-activating protein Rap1GAP uses a catalytic asparagine., Daumke O, Weyand M, Chakrabarti PP, Vetter IR, Wittinghofer A, Nature. 2004 May 13;429(6988):197-201. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15141215 15141215]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RPGP1_HUMAN RPGP1_HUMAN] GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1srq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1srq ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Chakrabarti, P.P.]]
+
[[Category: Chakrabarti PP]]
-
[[Category: Daumke, O.]]
+
[[Category: Daumke O]]
-
[[Category: Vetter, I.R.]]
+
[[Category: Vetter IR]]
-
[[Category: Weyand, M.]]
+
[[Category: Weyand M]]
-
[[Category: Wittinghofer, A.]]
+
[[Category: Wittinghofer A]]
-
[[Category: MPD]]
+
-
[[Category: SO4]]
+
-
[[Category: mixed alpha-beta]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:17:12 2007''
+

Current revision

Crystal Structure of the Rap1GAP catalytic domain

PDB ID 1srq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1srq"
Personal tools