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1qnw

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[[Image:1qnw.gif|left|200px]]
 
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==lectin II from Ulex europaeus==
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The line below this paragraph, containing "STRUCTURE_1qnw", creates the "Structure Box" on the page.
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<StructureSection load='1qnw' size='340' side='right'caption='[[1qnw]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qnw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ulex_europaeus Ulex europaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QNW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_1qnw| PDB=1qnw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qnw OCA], [https://pdbe.org/1qnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qnw RCSB], [https://www.ebi.ac.uk/pdbsum/1qnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qnw ProSAT]</span></td></tr>
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</table>
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'''LECTIN II FROM ULEX EUROPAEUS'''
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== Function ==
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[https://www.uniprot.org/uniprot/LEC2_ULEEU LEC2_ULEEU] Di-N-acetylchitobiose specific lectin.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Protein-carbohydrate interactions are the language of choice for inter- cellular communication. The legume lectins form a large family of homologous proteins that exhibit a wide variety of carbohydrate specificities. The legume lectin family is therefore highly suitable as a model system to study the structural principles of protein-carbohydrate recognition. Until now, structural data are only available for two specificity families: Man/Glc and Gal/GalNAc. No structural data are available for any of the fucose or chitobiose specific lectins.The crystal structure of Ulex europaeus (UEA-II) is the first of a legume lectin belonging to the chitobiose specificity group. The complexes with N-acetylglucosamine, galactose and fucosylgalactose show a promiscuous primary binding site capable of accommodating both N-acetylglucos amine or galactose in the primary binding site. The hydrogen bonding network in these complexes can be considered suboptimal, in agreement with the low affinities of these sugars. In the complexes with chitobiose, lactose and fucosyllactose this suboptimal hydrogen bonding network is compensated by extensive hydrophobic interactions in a Glc/GlcNAc binding subsite. UEA-II thus forms the first example of a legume lectin with a promiscuous binding site and illustrates the importance of hydrophobic interactions in protein-carbohydrate complexes. Together with other known legume lectin crystal structures, it shows how different specificities can be grafted upon a conserved structural framework.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/1qnw_consurf.spt"</scriptWhenChecked>
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1QNW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ulex_europaeus Ulex europaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNW OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural basis of carbohydrate recognition by lectin II from Ulex europaeus, a protein with a promiscuous carbohydrate-binding site., Loris R, De Greve H, Dao-Thi MH, Messens J, Imberty A, Wyns L, J Mol Biol. 2000 Aug 25;301(4):987-1002. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10966800 10966800]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qnw ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Ulex europaeus]]
[[Category: Ulex europaeus]]
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[[Category: Dao-Thi, M H.]]
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[[Category: Dao-Thi M-H]]
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[[Category: Greve, H De.]]
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[[Category: De Greve H]]
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[[Category: Imberty, A.]]
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[[Category: Imberty A]]
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[[Category: Loris, R.]]
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[[Category: Loris R]]
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[[Category: Messens, J.]]
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[[Category: Messens J]]
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[[Category: Wyns, L.]]
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[[Category: Wyns L]]
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[[Category: Carbohydrate binding]]
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[[Category: Lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:30:00 2008''
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Current revision

lectin II from Ulex europaeus

PDB ID 1qnw

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