1sy6

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(New page: 200px<br /> <applet load="1sy6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sy6, resolution 2.1&Aring;" /> '''Crystal Structure of...)
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[[Image:1sy6.gif|left|200px]]<br />
 
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<applet load="1sy6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sy6, resolution 2.1&Aring;" />
 
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'''Crystal Structure of CD3gammaepsilon Heterodimer in Complex with OKT3 Fab Fragment'''<br />
 
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==Overview==
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==Crystal Structure of CD3gammaepsilon Heterodimer in Complex with OKT3 Fab Fragment==
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The CD3 epsilon gamma heterodimer is essential for expression and function, of the T cell receptor. The crystal structure of the human CD3 epsilon, gamma heterodimer is described to 2.1-A resolution complexed with OKT3, a, therapeutic mAb that not only activates and tolerizes mature T cells but, also induces regulatory T cells. The mode of CD3 epsilon gamma, dimerization provides a general structural basis for CD3 assembly and maps, candidate T cell antigen receptor docking sites, including a duplicated, linear region rich in acidic residues that is unique to human CD3 epsilon., OKT3 binds to an atypically small area of CD3 epsilon and has a low, affinity for the isolated CD3 epsilon gamma heterodimer. The structure of, the OKT3/CD3 epsilon gamma complex has implications for T cell signaling, and therapeutic design.
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<StructureSection load='1sy6' size='340' side='right'caption='[[1sy6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sy6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SY6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sy6 OCA], [https://pdbe.org/1sy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sy6 RCSB], [https://www.ebi.ac.uk/pdbsum/1sy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sy6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6PJA7_MOUSE Q6PJA7_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sy/1sy6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sy6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The CD3 epsilon gamma heterodimer is essential for expression and function of the T cell receptor. The crystal structure of the human CD3 epsilon gamma heterodimer is described to 2.1-A resolution complexed with OKT3, a therapeutic mAb that not only activates and tolerizes mature T cells but also induces regulatory T cells. The mode of CD3 epsilon gamma dimerization provides a general structural basis for CD3 assembly and maps candidate T cell antigen receptor docking sites, including a duplicated linear region rich in acidic residues that is unique to human CD3 epsilon. OKT3 binds to an atypically small area of CD3 epsilon and has a low affinity for the isolated CD3 epsilon gamma heterodimer. The structure of the OKT3/CD3 epsilon gamma complex has implications for T cell signaling and therapeutic design.
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==About this Structure==
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Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3.,Kjer-Nielsen L, Dunstone MA, Kostenko L, Ely LK, Beddoe T, Mifsud NA, Purcell AW, Brooks AG, McCluskey J, Rossjohn J Proc Natl Acad Sci U S A. 2004 May 18;101(20):7675-80. Epub 2004 May 10. PMID:15136729<ref>PMID:15136729</ref>
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1SY6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SY6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3., Kjer-Nielsen L, Dunstone MA, Kostenko L, Ely LK, Beddoe T, Mifsud NA, Purcell AW, Brooks AG, McCluskey J, Rossjohn J, Proc Natl Acad Sci U S A. 2004 May 18;101(20):7675-80. Epub 2004 May 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15136729 15136729]
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</div>
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<div class="pdbe-citations 1sy6" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[CD3|CD3]]
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*[[CD3 3D structures|CD3 3D structures]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Beddoe T]]
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[[Category: Beddoe, T.]]
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[[Category: Brooks AG]]
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[[Category: Brooks, A.G.]]
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[[Category: Dunstone MA]]
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[[Category: Dunstone, M.A.]]
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[[Category: Ely LK]]
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[[Category: Ely, L.K.]]
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[[Category: Kjer-Nielsen L]]
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[[Category: Kjer-Nielsen, L.]]
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[[Category: Kostenko L]]
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[[Category: Kostenko, L.]]
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[[Category: McCluskey J]]
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[[Category: McCluskey, J.]]
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[[Category: Misfud NA]]
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[[Category: Misfud, N.A.]]
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[[Category: Purcell AW]]
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[[Category: Purcell, A.W.]]
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[[Category: Rossjohn J]]
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[[Category: Rossjohn, J.]]
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[[Category: cd3 epsilon]]
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[[Category: cd3 gamma]]
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[[Category: okt3 fab]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:18:13 2007''
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Current revision

Crystal Structure of CD3gammaepsilon Heterodimer in Complex with OKT3 Fab Fragment

PDB ID 1sy6

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