1qs2

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[[Image:1qs2.jpg|left|200px]]
 
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==CRYSTAL STRUCTURE OF VIP2 WITH NAD==
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The line below this paragraph, containing "STRUCTURE_1qs2", creates the "Structure Box" on the page.
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<StructureSection load='1qs2' size='340' side='right'caption='[[1qs2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qs2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QS2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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{{STRUCTURE_1qs2| PDB=1qs2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qs2 OCA], [https://pdbe.org/1qs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qs2 RCSB], [https://www.ebi.ac.uk/pdbsum/1qs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qs2 ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF VIP2 WITH NAD'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q844J9_BACTU Q844J9_BACTU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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A member of the Bacillus-produced vegetative insecticidal proteins (VIPs) possesses high specificity against the major insect pest, corn rootworms, and belongs to a class of binary toxins and regulators of biological pathways distinct from classical A-B toxins. The 1.5 A resolution crystal structure of the enzymatic ADP-ribosyltransferase component, VIP2, from Bacillus cereus reveals structurally homologous N- and C-terminal alpha/beta domains likely representing the entire class of binary toxins and implying evolutionary relationships between families of ADP-ribosylating toxins. The crystal structure of the kinetically trapped VIP2-NAD complex identifies the NAD binding cleft within the C-terminal enzymatic domain and provides a structural basis for understanding the targeting and catalysis of the medically and environmentally important binary toxins. These structures furthermore provide specific experimental results to help resolve paradoxes regarding the specific mechanism of ADP-ribosylation of actin by implicating ground state destabilization and nicotinamide product sequestration as the major driving forces for catalysis.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/1qs2_consurf.spt"</scriptWhenChecked>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QS2 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Evolution and mechanism from structures of an ADP-ribosylating toxin and NAD complex., Han S, Craig JA, Putnam CD, Carozzi NB, Tainer JA, Nat Struct Biol. 1999 Oct;6(10):932-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504727 10504727]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qs2 ConSurf].
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[[Category: Carozzi, N B.]]
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<div style="clear:both"></div>
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[[Category: Craig, J A.]]
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__TOC__
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[[Category: Han, S.]]
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</StructureSection>
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[[Category: Putnam, C D.]]
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[[Category: Bacillus cereus]]
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[[Category: Tainer, J A.]]
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[[Category: Large Structures]]
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[[Category: Alpha-beta protein]]
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[[Category: Carozzi NB]]
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[[Category: Binary toxin]]
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[[Category: Craig JA]]
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[[Category: Protein-nad complex]]
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[[Category: Han S]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:38:24 2008''
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[[Category: Putnam CD]]
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[[Category: Tainer JA]]

Current revision

CRYSTAL STRUCTURE OF VIP2 WITH NAD

PDB ID 1qs2

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