1qto

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[[Image:1qto.jpg|left|200px]]
 
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==1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS==
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The line below this paragraph, containing "STRUCTURE_1qto", creates the "Structure Box" on the page.
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<StructureSection load='1qto' size='340' side='right'caption='[[1qto]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qto]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QTO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qto OCA], [https://pdbe.org/1qto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qto RCSB], [https://www.ebi.ac.uk/pdbsum/1qto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qto ProSAT]</span></td></tr>
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{{STRUCTURE_1qto| PDB=1qto | SCENE= }}
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</table>
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== Function ==
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'''1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS'''
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[https://www.uniprot.org/uniprot/Q53793_9ACTN Q53793_9ACTN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Bleomycin (Bm)-binding protein, designated BLMA, which is a Bm resistance determinant from Bm-producing Streptomyces verticillus, was crystallized in a form suitable for X-ray diffraction analysis. The diffraction intensity data were collected up to a resolution of 1.5 A with a merging R-value of 0.054 at a completeness of 94 %. The BLMA structure, determined by the single isomorphous replacement method including the anomalous scattering effect (SIR-AS) at a resolution of 2.0 A, was refined at 1.5 A resolution. The final R-factor was 19.0 % and R(free) was 22.1 % including 91 water molecules. The crystal packing showed a dimer form, which was generated by arm exchange. The 1.5 A high-resolution experiment allowed an analysis of the side-chain disorder of BLMA. The structural comparison of BLMA with a homologous protein from Streptoalloteichus hindustanus, designated Shble protein, showed that a Ser100-Gly103 loop was farther from the groove, which is a Bm-binding site, in BLMA than in the Shble protein. Furthermore the hydrophobicity of the groove in BLMA is much lower than that in the Shble protein. The structural differences between these proteins may be responsible for the observation that a half-saturating concentration (K(1/2)) of Bm is higher for BLMA than for the Shble protein.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qt/1qto_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1QTO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTO OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qto ConSurf].
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The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus., Kawano Y, Kumagai T, Muta K, Matoba Y, Davies J, Sugiyama M, J Mol Biol. 2000 Jan 28;295(4):915-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10656800 10656800]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces verticillus]]
[[Category: Streptomyces verticillus]]
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[[Category: Davies, J.]]
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[[Category: Davies J]]
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[[Category: Kawano, Y.]]
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[[Category: Kawano Y]]
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[[Category: Kumagai, T.]]
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[[Category: Kumagai T]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Muta, K.]]
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[[Category: Muta K]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: Arm-exchange]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:41:31 2008''
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Current revision

1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS

PDB ID 1qto

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