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| - | [[Image:1quz.gif|left|200px]] | |
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| - | <!-- | + | ==Solution structure of the potassium channel scorpion toxin HSTX1== |
| - | The line below this paragraph, containing "STRUCTURE_1quz", creates the "Structure Box" on the page.
| + | <StructureSection load='1quz' size='340' side='right'caption='[[1quz]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1quz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Heterometrus_spinifer Heterometrus spinifer]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QUZ FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | --> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| - | {{STRUCTURE_1quz| PDB=1quz | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1quz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1quz OCA], [https://pdbe.org/1quz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1quz RCSB], [https://www.ebi.ac.uk/pdbsum/1quz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1quz ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/KAX63_HETSP KAX63_HETSP] Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.<ref>PMID:15498765</ref> <ref>PMID:18687312</ref> <ref>PMID:9359871</ref> |
| | + | <div style="background-color:#fffaf0;"> |
| | + | == Publication Abstract from PubMed == |
| | + | We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1, using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family, which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has the particularity to possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of the three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence on the global conformation of HsTX1. Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1. Thus, we propose that Tyr21, Lys23, Met25, and Asn26 are involved in the biological activity of HsTX1. As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage-gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels. |
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| - | '''Solution structure of the potassium channel scorpion toxin HSTX1'''
| + | Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1.,Savarin P, Romi-Lebrun R, Zinn-Justin S, Lebrun B, Nakajima T, Gilquin B, Menez A Protein Sci. 1999 Dec;8(12):2672-85. PMID:10631983<ref>PMID:10631983</ref> |
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| - | | + | |
| - | ==Overview==
| + | |
| - | We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1, using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family, which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has the particularity to possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of the three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence on the global conformation of HsTX1. Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1. Thus, we propose that Tyr21, Lys23, Met25, and Asn26 are involved in the biological activity of HsTX1. As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage-gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels.
| + | |
| | | | |
| - | ==About this Structure==
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | 1QUZ is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QUZ OCA].
| + | </div> |
| | + | <div class="pdbe-citations 1quz" style="background-color:#fffaf0;"></div> |
| | | | |
| - | ==Reference== | + | ==See Also== |
| - | Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1., Savarin P, Romi-Lebrun R, Zinn-Justin S, Lebrun B, Nakajima T, Gilquin B, Menez A, Protein Sci. 1999 Dec;8(12):2672-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10631983 10631983]
| + | *[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]] |
| - | [[Category: Single protein]] | + | == References == |
| - | [[Category: Gilquin, B.]] | + | <references/> |
| - | [[Category: Lebrun, B.]] | + | __TOC__ |
| - | [[Category: Menez, A.]] | + | </StructureSection> |
| - | [[Category: Nakajima, T.]] | + | [[Category: Heterometrus spinifer]] |
| - | [[Category: Romi-Lebrun, R.]] | + | [[Category: Large Structures]] |
| - | [[Category: Savarin, P.]] | + | [[Category: Gilquin B]] |
| - | [[Category: Zinn-Justin, S.]] | + | [[Category: Lebrun B]] |
| - | [[Category: Alpha beta]]
| + | [[Category: Menez A]] |
| - | [[Category: Molecular modeling]]
| + | [[Category: Nakajima T]] |
| - | [[Category: Nmr]]
| + | [[Category: Romi-Lebrun R]] |
| - | [[Category: Potassium channel]]
| + | [[Category: Savarin P]] |
| - | [[Category: Scorpion toxin]]
| + | [[Category: Zinn-Justin S]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:44:04 2008''
| + | |
| Structural highlights
Function
KAX63_HETSP Potently blocks voltage-gated potassium channels Kv1.1/KCNA1 and Kv1.3/KCNA3. Mildly blocks intermediate (IK) conductance calcium-activated potassium channels (KCa3.1/KCNN4) and ERG1/Kv11.1/KCNH2.[1] [2] [3]
Publication Abstract from PubMed
We have determined the three-dimensional structure of the potassium channel inhibitor HsTX1, using nuclear magnetic resonance and molecular modeling. This protein belongs to the scorpion short toxin family, which essentially contains potassium channel blockers of 29 to 39 amino acids and three disulfide bridges. It is highly active on voltage-gated Kv1.3 potassium channels. Furthermore, it has the particularity to possess a fourth disulfide bridge. We show that HsTX1 has a fold similar to that of the three-disulfide-bridged toxins and conserves the hydrophobic core found in the scorpion short toxins. Thus, the fourth bridge has no influence on the global conformation of HsTX1. Most residues spatially analogous to those interacting with voltage-gated potassium channels in the three-disulfide-bridged toxins are conserved in HsTX1. Thus, we propose that Tyr21, Lys23, Met25, and Asn26 are involved in the biological activity of HsTX1. As an additional positively charged residue is always spatially close to the aromatic residue in toxins blocking the voltage-gated potassium channels, and as previous mutagenesis experiments have shown the critical role played by the C-terminus in HsTX1, we suggest that Arg33 is also important for the activity of the four disulfide-bridged toxin. Docking calculations confirm that, if Lys23 and Met25 interact with the GYGDMH motif of Kv1.3, Arg33 can contact Asp386 and, thus, play the role of the additional positively charged residue of the toxin functional site. This original configuration of the binding site of HsTX1 for Kv1.3, if confirmed experimentally, offers new structural possibilities for the construction of a molecule blocking the voltage-gated potassium channels.
Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1.,Savarin P, Romi-Lebrun R, Zinn-Justin S, Lebrun B, Nakajima T, Gilquin B, Menez A Protein Sci. 1999 Dec;8(12):2672-85. PMID:10631983[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Regaya I, Beeton C, Ferrat G, Andreotti N, Darbon H, De Waard M, Sabatier JM. Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins. J Biol Chem. 2004 Dec 31;279(53):55690-6. Epub 2004 Oct 21. PMID:15498765 doi:M410055200
- ↑ Abdel-Mottaleb Y, Corzo G, Martin-Eauclaire MF, Satake H, Ceard B, Peigneur S, Nambaru P, Bougis PE, Possani LD, Tytgat J. A common "hot spot" confers hERG blockade activity to alpha-scorpion toxins affecting K+ channels. Biochem Pharmacol. 2008 Sep 15;76(6):805-15. doi: 10.1016/j.bcp.2008.07.008. Epub, 2008 Jul 18. PMID:18687312 doi:http://dx.doi.org/10.1016/j.bcp.2008.07.008
- ↑ Lebrun B, Romi-Lebrun R, Martin-Eauclaire MF, Yasuda A, Ishiguro M, Oyama Y, Pongs O, Nakajima T. A four-disulphide-bridged toxin, with high affinity towards voltage-gated K+ channels, isolated from Heterometrus spinnifer (Scorpionidae) venom. Biochem J. 1997 Nov 15;328 ( Pt 1):321-7. PMID:9359871
- ↑ Savarin P, Romi-Lebrun R, Zinn-Justin S, Lebrun B, Nakajima T, Gilquin B, Menez A. Structural and functional consequences of the presence of a fourth disulfide bridge in the scorpion short toxins: solution structure of the potassium channel inhibitor HsTX1. Protein Sci. 1999 Dec;8(12):2672-85. PMID:10631983
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