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1qxh

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[[Image:1qxh.jpg|left|200px]]
 
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==Crystal Structure of Escherichia coli Thiol Peroxidase in the Oxidized State==
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The line below this paragraph, containing "STRUCTURE_1qxh", creates the "Structure Box" on the page.
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<StructureSection load='1qxh' size='340' side='right'caption='[[1qxh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qxh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxh OCA], [https://pdbe.org/1qxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qxh RCSB], [https://www.ebi.ac.uk/pdbsum/1qxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxh ProSAT]</span></td></tr>
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{{STRUCTURE_1qxh| PDB=1qxh | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TPX_ECOLI TPX_ECOLI] Has antioxidant activity. Could remove peroxides or H(2)O(2) within the catalase- and peroxidase-deficient periplasmic space.[HAMAP-Rule:MF_00269]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qxh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qxh ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Escherichia coli Thiol Peroxidase in the Oxidized State'''
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==See Also==
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*[[Thiol peroxidase 3D structures|Thiol peroxidase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Thioredoxin-dependent thiol peroxidase (Tpx) from Escherichia coli represents a group of antioxidant enzymes that are widely distributed in pathogenic bacterial species and which belong to the peroxiredoxin (Prx) family. Bacterial Tpxs are unique in that the location of the resolving cysteine (CR) is different from those of other Prxs. E. coli Tpx (EcTpx) shows substrate specificity toward alkyl hydroperoxides over H2O2 and is the most potent reductant of alkyl hydroperoxides surpassing AhpC and BCP, the other E. coli Prx members. Here, we present the crystal structure of EcTpx in the oxidized state determined at 2.2-A resolution. The structure revealed that Tpxs are the second type of atypical 2-Cys Prxs with an intramolecular disulfide bond formed between the peroxidatic (CP, Cys61) and resolving (Cys95) cysteine residues. The extraordinarily long N-terminal chain of EcTpx folds into a beta-hairpin making the overall structure very compact. Modeling suggests that, in atypical 2-Cys Prxs, the CR-loop as well as the CP-loop may alternately assume the fully folded or locally unfolded conformation depending on redox states, as does the CP-loop in typical 2-Cys Prxs. EcTpx exists as a dimer stabilized by hydrogen bonds. Its substrate binding site extends to the dimer interface. A modeled structure of the reduced EcTpx in complex with 15-hydroperoxyeicosatetraenoic acid suggests that the size and shape of the binding site are particularly suited for long fatty acid hydroperoxides consistent with its greater reactivity.
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==About this Structure==
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1QXH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXH OCA].
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==Reference==
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Crystal structure of Escherichia coli thiol peroxidase in the oxidized state: insights into intramolecular disulfide formation and substrate binding in atypical 2-Cys peroxiredoxins., Choi J, Choi S, Choi J, Cha MK, Kim IH, Shin W, J Biol Chem. 2003 Dec 5;278(49):49478-86. Epub 2003 Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14506251 14506251]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Choi, J.]]
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[[Category: Choi J]]
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[[Category: Choi, S.]]
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[[Category: Choi S]]
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[[Category: Shin, W.]]
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[[Category: Shin W]]
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[[Category: Antioxidant enzyme]]
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[[Category: Peroxiredoxin]]
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[[Category: Scavengase p20]]
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[[Category: Thiol peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:49:12 2008''
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Current revision

Crystal Structure of Escherichia coli Thiol Peroxidase in the Oxidized State

PDB ID 1qxh

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