1r0s

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[[Image:1r0s.jpg|left|200px]]
 
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==Crystal structure of ADP-ribosyl cyclase Glu179Ala mutant==
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The line below this paragraph, containing "STRUCTURE_1r0s", creates the "Structure Box" on the page.
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<StructureSection load='1r0s' size='340' side='right'caption='[[1r0s]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1r0s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R0S FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0s OCA], [https://pdbe.org/1r0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r0s RCSB], [https://www.ebi.ac.uk/pdbsum/1r0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r0s ProSAT]</span></td></tr>
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{{STRUCTURE_1r0s| PDB=1r0s | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NADA_APLCA NADA_APLCA] Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for calcium mobilization from endoplasmic reticulum. Also has cADPr hydrolase activity.<ref>PMID:11829748</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/1r0s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r0s ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of ADP-ribosyl cyclase Glu179Ala mutant'''
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==See Also==
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*[[Cluster of Differentiation CD38|Cluster of Differentiation CD38]]
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== References ==
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==Overview==
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<references/>
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ADP-ribosyl cyclase catalyzes the elimination of nicotinamide from NAD and cyclization to cADPR, a known second messenger in cellular calcium signaling pathways. We have determined to 2.0 A resolution the structure of Aplysia cyclase with ribose-5-phosphate bound covalently at C3' and with the base exchange substrate (BES), pyridylcarbinol, bound to the active site. In addition, further refinement at 2.4 A resolution of the structure of nicotinamide-bound cyclase, which was previously reported, reveals that ribose-5-phosphate is also covalently bound in this structure, and a second nicotinamide site was identified. The structures of native and mutant Glu179Ala cyclase were also solved to 1.7 and 2.0 A respectively. It is proposed that the second nicotinamide site serves to promote cyclization by clearing the active site of the nicotinamide byproduct. Moreover, a ribosylation mechanism can be proposed in which the cyclization reaction proceeds through a covalently bound intermediate.
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__TOC__
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</StructureSection>
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==About this Structure==
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1R0S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0S OCA].
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==Reference==
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ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate., Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q, Structure. 2004 Mar;12(3):477-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016363 15016363]
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Graeff, R.]]
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[[Category: Graeff R]]
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[[Category: Hao, Q.]]
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[[Category: Hao Q]]
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[[Category: Kriksunov, I A.]]
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[[Category: Kriksunov IA]]
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[[Category: Lee, H C.]]
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[[Category: Lee HC]]
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[[Category: Love, M L.]]
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[[Category: Love ML]]
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[[Category: Munshi, C.]]
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[[Category: Munshi C]]
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[[Category: Szebenyi, D M.E.]]
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[[Category: Szebenyi DME]]
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[[Category: Thiel, D J.]]
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[[Category: Thiel DJ]]
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[[Category: Adp-ribosyl cyclase]]
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[[Category: Ca2+ signalling]]
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[[Category: Cyclic adp-ribose]]
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[[Category: Naadp]]
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[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:56:06 2008''
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Current revision

Crystal structure of ADP-ribosyl cyclase Glu179Ala mutant

PDB ID 1r0s

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