1r0v

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[[Image:1r0v.jpg|left|200px]]
 
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==Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21212 space group==
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The line below this paragraph, containing "STRUCTURE_1r0v", creates the "Structure Box" on the page.
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<StructureSection load='1r0v' size='340' side='right'caption='[[1r0v]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1r0v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R0V FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0v OCA], [https://pdbe.org/1r0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r0v RCSB], [https://www.ebi.ac.uk/pdbsum/1r0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r0v ProSAT]</span></td></tr>
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{{STRUCTURE_1r0v| PDB=1r0v | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ENDA_ARCFU ENDA_ARCFU] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/1r0v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r0v ConSurf].
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<div style="clear:both"></div>
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'''Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21212 space group'''
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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RNA-splicing endonuclease is responsible for the excision of introns in transfer RNA and archaeal ribosomal RNAs. The archaeal form of the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-paired helix, known as the bulge-helix-bulge (BHB) motif. A crystal structure of the RNA-splicing endonuclease from Archaeoglobus fulgidus (AF) has been reported previously at 2.8 A. A truncated but fully active form of AF endonuclease that lacks the N-terminal domain was expressed and crystallized in an orthorhombic space group with two dimers in the asymmetric unit. The calculated native Patterson map suggests strong pseudo-face-centering characteristics, which lead to incorrect space-group assignment by the autoindexing program. The correct space group was determined to be P2(1)2(1)2 after reindexing. The structure was solved using molecular replacement and was refined to 2.0 A. The truncated AF endonuclease structure is essentially identical to the corresponding portion of the wild-type AF endonuclease structure in space group P4(3)2(1)2 as reported previously, with the exception of loop L9, which differs owing to different crystallographic packing. These results confirm the previously described structural features of dimeric splicing endonuclease.
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Li H]]
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1R0V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0V OCA].
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[[Category: Zhang Y]]
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==Reference==
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Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2(1)2(1)2., Zhang Y, Li H, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):447-52. Epub 2004, Feb 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14993668 14993668]
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[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: TRNA-intron endonuclease]]
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[[Category: Li, H.]]
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[[Category: Zhang, Y.]]
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[[Category: Endonuclease]]
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[[Category: Rna splicing]]
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[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:56:15 2008''
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Current revision

Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21212 space group

PDB ID 1r0v

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