1r22

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:19, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1r22.gif|left|200px]]
 
-
<!--
+
==Crystal structure of the cyanobacterial metallothionein repressor SmtB (C14S/C61S/C121S mutant) in the Zn2alpha5-form==
-
The line below this paragraph, containing "STRUCTURE_1r22", creates the "Structure Box" on the page.
+
<StructureSection load='1r22' size='340' side='right'caption='[[1r22]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1r22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R22 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_1r22| PDB=1r22 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r22 OCA], [https://pdbe.org/1r22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r22 RCSB], [https://www.ebi.ac.uk/pdbsum/1r22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r22 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Crystal structure of the cyanobacterial metallothionein repressor SmtB (C14S/C61S/C121S mutant) in the Zn2alpha5-form'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/SMTB_SYNE7 SMTB_SYNE7] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.<ref>PMID:12146943</ref> <ref>PMID:12407207</ref> <ref>PMID:8871549</ref>
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The origin of metal ion selectivity by members of the SmtB/ArsR family of bacterial metal-sensing transcriptional repressors and the mechanism of negative allosteric regulation of DNA binding is poorly understood. Here, we report that two homologous zinc sensors, Staphylococcus aureus CzrA and cyanobacterial SmtB, are "winged" helix homodimeric DNA-binding proteins that bind Zn(II) to a pair of tetrahedral, interhelical binding sites, with two ligands derived from the alpha5 helix of one subunit, Asp84 O(delta1) (Asp104 in SmtB), His86 N(delta1) (His106), and two derived from the alpha5 helix of the other, His97' N(delta1) (His117') and His100' N(epsilon2) (Glu120'). Formation of the metal chelate drives a quaternary structural switch mediated by an intersubunit hydrogen-binding network that originates with the non-liganding N(epsilon2) face of His97 in CzrA (His117 in SmtB) that stabilizes a low-affinity, DNA-binding conformation. The structure of the Zn(1) SmtB homodimer shows that both metal-binding sites of the dimer must be occupied for the quaternary structural switch to occur. Thus, a critical zinc-ligating histidine residue obligatorily couples formation of the metal-sensing coordination chelate to changes in the conformation and dynamics of the putative DNA-binding helices.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r22_consurf.spt"</scriptWhenChecked>
-
1R22 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R22 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins., Eicken C, Pennella MA, Chen X, Koshlap KM, VanZile ML, Sacchettini JC, Giedroc DP, J Mol Biol. 2003 Oct 31;333(4):683-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14568530 14568530]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r22 ConSurf].
-
[[Category: Single protein]]
+
<div style="clear:both"></div>
-
[[Category: Synechococcus sp.]]
+
== References ==
-
[[Category: Chen, X.]]
+
<references/>
-
[[Category: Eicken, C.]]
+
__TOC__
-
[[Category: Giedroc, D P.]]
+
</StructureSection>
-
[[Category: Koshlap, K M.]]
+
[[Category: Large Structures]]
-
[[Category: Pennella, M A.]]
+
[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
-
[[Category: Sacchettini, J C.]]
+
[[Category: Chen X]]
-
[[Category: VanZile, M L.]]
+
[[Category: Eicken C]]
-
[[Category: Dna binding protein]]
+
[[Category: Giedroc DP]]
-
[[Category: Transcriptional regulation]]
+
[[Category: Koshlap KM]]
-
[[Category: Winged hth protein]]
+
[[Category: Pennella MA]]
-
[[Category: Zinc]]
+
[[Category: Sacchettini JC]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:58:54 2008''
+
[[Category: VanZile ML]]

Current revision

Crystal structure of the cyanobacterial metallothionein repressor SmtB (C14S/C61S/C121S mutant) in the Zn2alpha5-form

PDB ID 1r22

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r22"
Personal tools