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1tgj

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(New page: 200px<br /> <applet load="1tgj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tgj, resolution 2.0&Aring;" /> '''HUMAN TRANSFORMING G...)
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[[Image:1tgj.gif|left|200px]]<br />
 
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<applet load="1tgj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tgj, resolution 2.0&Aring;" />
 
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'''HUMAN TRANSFORMING GROWTH FACTOR-BETA 3, CRYSTALLIZED FROM DIOXANE'''<br />
 
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==Overview==
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==HUMAN TRANSFORMING GROWTH FACTOR-BETA 3, CRYSTALLIZED FROM DIOXANE==
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Transforming growth factors beta belong to a group of cytokines that, control cellular proliferation and differentiation. Five isoforms are, known that share approximately 75% sequence identity, but exert different, biological activities. The structure of TGF-beta 3 was solved by X-ray, crystallography and refined to a final R-factor of 17.5% at 2.0 A, resolution. Comparison with the structure of TGF-beta 2 (Schlunegger MP, Grutter MG, 1992, Nature 358:430-434; Daopin S, Piez KA, Ogawa Y, Davies, DR, 1992, Science 257:369-373) reveals a virtually identical central core., Differences exist in the conformations of the N-terminal alpha-helix and, in the beta-sheet loops. In TGF-beta 3, the N-terminal alpha-helix has, moved approximately 1 A away from the central core. This movement can be, correlated with the mutation of Leu 17 to Val and Ala 47 to Pro in, TGF-beta 3. The beta-sheet loops rotate as a rigid body 9 degrees around, an axis that runs approximately parallel to the dimer axis. If these, differences are recognized by the TGF-beta receptors, they might account, for the individual cellular responses. A molecule of the precipitating, agent dioxane is bound in a crystal contact, forming a hydrogen bond with, Trp 32. This dioxane may occupy a carbohydrate-binding site, because, dioxane possesses some structural similarity with a carbohydrate. The, dioxane is in contact with two tryptophans, which are often involved in, carbohydrate recognition.
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<StructureSection load='1tgj' size='340' side='right'caption='[[1tgj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1tgj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TGJ FirstGlance]. <br>
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Known disease associated with this structure: Arrhythmogenic right ventricular dysplasia 1 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=190230 190230]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tgj OCA], [https://pdbe.org/1tgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tgj RCSB], [https://www.ebi.ac.uk/pdbsum/1tgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tgj ProSAT]</span></td></tr>
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1TGJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with DIO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TGJ OCA].
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</table>
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== Disease ==
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==Reference==
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[https://www.uniprot.org/uniprot/TGFB3_HUMAN TGFB3_HUMAN] Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:[https://omim.org/entry/107970 107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.<ref>PMID:15639475</ref>
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The crystal structure of TGF-beta 3 and comparison to TGF-beta 2: implications for receptor binding., Mittl PR, Priestle JP, Cox DA, McMaster G, Cerletti N, Grutter MG, Protein Sci. 1996 Jul;5(7):1261-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8819159 8819159]
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== Function ==
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[https://www.uniprot.org/uniprot/TGFB3_HUMAN TGFB3_HUMAN] Involved in embryogenesis and cell differentiation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/1tgj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tgj ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gruetter, M.G.]]
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[[Category: Gruetter MG]]
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[[Category: Mittl, P.R.E.]]
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[[Category: Mittl PRE]]
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[[Category: Priestle, J.P.]]
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[[Category: Priestle JP]]
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[[Category: DIO]]
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[[Category: glycoprotein]]
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[[Category: growth factor]]
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[[Category: mitogen]]
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[[Category: signal]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:24:40 2007''
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Current revision

HUMAN TRANSFORMING GROWTH FACTOR-BETA 3, CRYSTALLIZED FROM DIOXANE

PDB ID 1tgj

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