1twm

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(New page: 200px<br /> <applet load="1twm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1twm, resolution 2.26&Aring;" /> '''Interleukin-1 Beta ...)
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[[Image:1twm.gif|left|200px]]<br />
 
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<applet load="1twm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1twm, resolution 2.26&Aring;" />
 
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'''Interleukin-1 Beta Mutant F146Y'''<br />
 
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==Overview==
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==Interleukin-1 Beta Mutant F146Y==
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The structural and energetic consequences of modifications to the, hydrophobic cavity of interleukin 1-beta (IL-1beta) are described., Previous reports demonstrated that the entirely hydrophobic cavity of, IL-1beta contains positionally disordered water. To gain a better, understanding of the nature of this cavity and the water therein, a number, of mutant proteins were constructed by site-directed mutagenesis, designed, to result in altered hydrophobicity of the cavity. These mutations involve, the replacement of specific phenylalanine residues, which circumscribe the, cavity, with tyrosine, tryptophan, leucine and isoleucine. Using, differential scanning calorimetry to determine the relative stabilities of, the wild-type and mutant proteins, we found all of the mutants to be, destabilizing. X-ray crystallography was used to identify the structural, consequences of the mutations. No clear correlation between the, hydrophobicities of the specific side-chains introduced and the resulting, stabilities was found.
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<StructureSection load='1twm' size='340' side='right'caption='[[1twm]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1twm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1twm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twm OCA], [https://pdbe.org/1twm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1twm RCSB], [https://www.ebi.ac.uk/pdbsum/1twm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1twm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1twm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1twm ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Gastric cancer risk after H. pylori infection OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147720 147720]]
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*[[Interleukin 3D structures|Interleukin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1TWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TWM OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structural and energetic consequences of mutations in a solvated hydrophobic cavity., Adamek DH, Guerrero L, Blaber M, Caspar DL, J Mol Biol. 2005 Feb 11;346(1):307-18. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15663946 15663946]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Adamek, D.H.]]
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[[Category: Adamek DH]]
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[[Category: Capsar, D.L.]]
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[[Category: Capsar DL]]
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[[Category: hydrophobic cavity]]
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[[Category: hydrophobicity]]
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[[Category: solvation]]
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[[Category: water]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:28:48 2007''
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Current revision

Interleukin-1 Beta Mutant F146Y

PDB ID 1twm

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