1txu

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(New page: 200px<br /> <applet load="1txu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1txu, resolution 2.35&Aring;" /> '''Crystal Structure o...)
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[[Image:1txu.gif|left|200px]]<br />
 
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<applet load="1txu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1txu, resolution 2.35&Aring;" />
 
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'''Crystal Structure of the Vps9 Domain of Rabex-5'''<br />
 
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==Overview==
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==Crystal Structure of the Vps9 Domain of Rabex-5==
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The Rab5 GTPase, an essential regulator of endocytosis and endosome, biogenesis, is activated by guanine-nucleotide exchange factors (GEFs), that contain a Vps9 domain. Here, we show that the catalytic core of the, Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain, stabilized by an indispensable helical bundle. A family-wide analysis of, Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases., Conserved exchange determinants map to a common surface of the Vps9, domain, which recognizes invariant aromatic residues in the switch regions, of Rab GTPases and selects for the Rab5 subfamily by requiring a small, nonacidic residue preceding a critical phenylalanine in the switch I, region. These and other observations reveal unexpected similarity with the, Arf exchange site in the Sec7 domain.
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<StructureSection load='1txu' size='340' side='right'caption='[[1txu]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1txu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txu OCA], [https://pdbe.org/1txu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txu RCSB], [https://www.ebi.ac.uk/pdbsum/1txu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RABX5_HUMAN RABX5_HUMAN] Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity).<ref>PMID:9323142</ref> <ref>PMID:11452015</ref> <ref>PMID:15339665</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Rab5 GTPase, an essential regulator of endocytosis and endosome biogenesis, is activated by guanine-nucleotide exchange factors (GEFs) that contain a Vps9 domain. Here, we show that the catalytic core of the Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain stabilized by an indispensable helical bundle. A family-wide analysis of Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases. Conserved exchange determinants map to a common surface of the Vps9 domain, which recognizes invariant aromatic residues in the switch regions of Rab GTPases and selects for the Rab5 subfamily by requiring a small nonacidic residue preceding a critical phenylalanine in the switch I region. These and other observations reveal unexpected similarity with the Arf exchange site in the Sec7 domain.
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==About this Structure==
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Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5.,Delprato A, Merithew E, Lambright DG Cell. 2004 Sep 3;118(5):607-17. PMID:15339665<ref>PMID:15339665</ref>
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1TXU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TXU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5., Delprato A, Merithew E, Lambright DG, Cell. 2004 Sep 3;118(5):607-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15339665 15339665]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1txu" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Delprato, A.]]
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[[Category: Lambright, D.G.]]
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[[Category: Merithew, E.]]
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[[Category: MG]]
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[[Category: gef]]
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[[Category: rab5 guanine-nucleotide exchange factor]]
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[[Category: vps9 domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:29:14 2007''
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==See Also==
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*[[Rab5 GDP/GTP exchange factor|Rab5 GDP/GTP exchange factor]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Delprato A]]
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[[Category: Lambright DG]]
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[[Category: Merithew E]]

Current revision

Crystal Structure of the Vps9 Domain of Rabex-5

PDB ID 1txu

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