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| - | [[Image:1rh6.gif|left|200px]] | |
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| - | <!--
| + | ==Bacteriophage Lambda Excisionase (Xis)-DNA Complex== |
| - | The line below this paragraph, containing "STRUCTURE_1rh6", creates the "Structure Box" on the page.
| + | <StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH6 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1rh6| PDB=1rh6 | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''Bacteriophage Lambda Excisionase (Xis)-DNA Complex'''
| + | [https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site. |
| - | | + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==Overview== | + | [[Category: Escherichia virus Lambda]] |
| - | The excisionase (Xis) protein from bacteriophage lambda is the best characterized member of a large family of recombination directionality factors that control integrase-mediated DNA rearrangements. It triggers phage excision by cooperatively binding to sites X1 and X2 within the phage, bending DNA significantly and recruiting the phage-encoded integrase (Int) protein to site P2. We have determined the co-crystal structure of Xis with its X2 DNA-binding site at 1.7A resolution. Xis forms a unique winged-helix motif that interacts with the major and minor grooves of its binding site using an alpha-helix and an ordered beta-hairpin (wing), respectively. Recognition is achieved through an elaborate water-mediated hydrogen-bonding network at the major groove interface, while the preformed hairpin forms largely non-specific interactions with the minor groove. The structure of the complex provides insights into how Xis recruits Int cooperatively, and suggests a plausible mechanism by which it may distort longer DNA fragments significantly. It reveals a surface on the protein that is likely to mediate Xis-Xis interactions required for its cooperative binding to DNA.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Cascio D]] |
| - | ==About this Structure== | + | [[Category: Clubb RT]] |
| - | 1RH6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA].
| + | [[Category: Johnson RC]] |
| - | | + | [[Category: Sam MD]] |
| - | ==Reference== | + | |
| - | Crystal structure of the excisionase-DNA complex from bacteriophage lambda., Sam MD, Cascio D, Johnson RC, Clubb RT, J Mol Biol. 2004 Apr 23;338(2):229-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15066428 15066428]
| + | |
| - | [[Category: Enterobacteria phage lambda]] | + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Cascio, D.]] | + | |
| - | [[Category: Clubb, R T.]] | + | |
| - | [[Category: Johnson, R C.]] | + | |
| - | [[Category: Sam, M D.]] | + | |
| - | [[Category: Winged -helix protein]] | + | |
| - | [[Category: Dna architectural protein]] | + | |
| - | [[Category: Phage excision]] | + | |
| - | [[Category: Protein-dna complex]] | + | |
| - | [[Category: Site-specific dna recombination]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:29:40 2008''
| + | |
