1rk4

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[[Image:1rk4.jpg|left|200px]]
 
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==Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1==
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The line below this paragraph, containing "STRUCTURE_1rk4", creates the "Structure Box" on the page.
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<StructureSection load='1rk4' size='340' side='right'caption='[[1rk4]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1rk4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RK4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.792&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rk4 OCA], [https://pdbe.org/1rk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rk4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rk4 ProSAT]</span></td></tr>
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{{STRUCTURE_1rk4| PDB=1rk4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CLIC1_HUMAN CLIC1_HUMAN] Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.<ref>PMID:9139710</ref> <ref>PMID:10834939</ref> <ref>PMID:11195932</ref> <ref>PMID:11940526</ref> <ref>PMID:11978800</ref> <ref>PMID:11551966</ref> <ref>PMID:14613939</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rk4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rk4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in both soluble and integral membrane forms. The structure of the soluble form of CLIC1 is typical of a soluble glutathione S-transferase superfamily protein but contains a glutaredoxin-like active site. In this study we show that on oxidation CLIC1 undergoes a reversible transition from a monomeric to a non-covalent dimeric state due to the formation of an intramolecular disulfide bond (Cys-24-Cys-59). We have determined the crystal structure of this oxidized state and show that a major structural transition has occurred, exposing a large hydrophobic surface, which forms the dimer interface. The oxidized CLIC1 dimer maintains its ability to form chloride ion channels in artificial bilayers and vesicles, whereas a reducing environment prevents the formation of ion channels by CLIC1. Mutational studies show that both Cys-24 and Cys-59 are required for channel activity.
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'''Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1'''
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The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition.,Littler DR, Harrop SJ, Fairlie WD, Brown LJ, Pankhurst GJ, Pankhurst S, DeMaere MZ, Campbell TJ, Bauskin AR, Tonini R, Mazzanti M, Breit SN, Curmi PM J Biol Chem. 2004 Mar 5;279(10):9298-305. Epub 2003 Nov 12. PMID:14613939<ref>PMID:14613939</ref>
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==Overview==
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Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in both soluble and integral membrane forms. The structure of the soluble form of CLIC1 is typical of a soluble glutathione S-transferase superfamily protein but contains a glutaredoxin-like active site. In this study we show that on oxidation CLIC1 undergoes a reversible transition from a monomeric to a non-covalent dimeric state due to the formation of an intramolecular disulfide bond (Cys-24-Cys-59). We have determined the crystal structure of this oxidized state and show that a major structural transition has occurred, exposing a large hydrophobic surface, which forms the dimer interface. The oxidized CLIC1 dimer maintains its ability to form chloride ion channels in artificial bilayers and vesicles, whereas a reducing environment prevents the formation of ion channels by CLIC1. Mutational studies show that both Cys-24 and Cys-59 are required for channel activity.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1RK4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK4 OCA].
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</div>
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<div class="pdbe-citations 1rk4" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition., Littler DR, Harrop SJ, Fairlie WD, Brown LJ, Pankhurst GJ, Pankhurst S, DeMaere MZ, Campbell TJ, Bauskin AR, Tonini R, Mazzanti M, Breit SN, Curmi PM, J Biol Chem. 2004 Mar 5;279(10):9298-305. Epub 2003 Nov 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14613939 14613939]
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bauskin, A R.]]
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[[Category: Bauskin AR]]
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[[Category: Breit, S N.]]
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[[Category: Breit SN]]
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[[Category: Brown, L J.]]
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[[Category: Brown LJ]]
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[[Category: Campbell, T J.]]
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[[Category: Campbell TJ]]
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[[Category: Curmi, P M.]]
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[[Category: Curmi PM]]
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[[Category: DeMaere, M Z.]]
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[[Category: DeMaere MZ]]
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[[Category: Fairlie, W D.]]
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[[Category: Fairlie WD]]
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[[Category: Harrop, S J.]]
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[[Category: Harrop SJ]]
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[[Category: Littler, D R.]]
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[[Category: Littler DR]]
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[[Category: Mazzanti, M.]]
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[[Category: Mazzanti M]]
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[[Category: Pankhurst, G J.]]
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[[Category: Pankhurst GJ]]
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[[Category: Pankhurst, S.]]
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[[Category: Pankhurst S]]
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[[Category: Tonini, R.]]
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[[Category: Tonini R]]
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[[Category: Chloride ion channel]]
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[[Category: Glutathione-s-tranferase superfamily]]
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[[Category: Redox-controlled structural transition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:35:45 2008''
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Current revision

Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1

PDB ID 1rk4

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