1rk8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:23, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1rk8.gif|left|200px]]
 
-
<!--
+
==Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex==
-
The line below this paragraph, containing "STRUCTURE_1rk8", creates the "Structure Box" on the page.
+
<StructureSection load='1rk8' size='340' side='right'caption='[[1rk8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1rk8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RK8 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-
{{STRUCTURE_1rk8| PDB=1rk8 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rk8 OCA], [https://pdbe.org/1rk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1rk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rk8 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/MGN_DROME MGN_DROME] Participates in the bidirectional intercellular signaling between the posterior follicle cells and oocyte to establish spatial coordinates that induces axis formation. Complex with tsu is essential for cytoplasmic localization of oskar in the posterior pole of oocytes. Required for the polarization of the oocyte microtubule cytoskeleton.<ref>PMID:8026338</ref> <ref>PMID:9272960</ref> <ref>PMID:9210377</ref> <ref>PMID:12730685</ref>
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago-Y14 heterodimer is a core component of the EJC. Recently, the protein PYM has been identified as an interacting partner of Mago-Y14. Here we show that PYM is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. PYM interacts directly with Mago-Y14 by means of its N-terminal domain. The crystal structure of the Drosophila ternary complex at 1.9 A resolution reveals that PYM binds Mago and Y14 simultaneously, capping their heterodimerization interface at conserved surface residues. Formation of this ternary complex is also observed with the human proteins. Mago residues involved in the interaction with PYM have been implicated in nonsense-mediated mRNA decay (NMD). Consistently, human PYM is active in NMD tethering assays. Together, these data suggest a role for PYM in NMD.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rk8_consurf.spt"</scriptWhenChecked>
-
1RK8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK8 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex., Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E, EMBO Rep. 2004 Mar;5(3):304-10. Epub 2004 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14968132 14968132]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rk8 ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Bono, F.]]
+
[[Category: Bono F]]
-
[[Category: Conti, E.]]
+
[[Category: Conti E]]
-
[[Category: Ebert, J.]]
+
[[Category: Ebert J]]
-
[[Category: Guettler, T.]]
+
[[Category: Guettler T]]
-
[[Category: Izaurralde, E.]]
+
[[Category: Izaurralde E]]
-
[[Category: Mrna processing]]
+
-
[[Category: Nmd]]
+
-
[[Category: Oskar mrna localization]]
+
-
[[Category: Rbd]]
+
-
[[Category: Rrm]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:35:56 2008''
+

Current revision

Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex

PDB ID 1rk8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rk8"
Personal tools