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1rmn

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[[Image:1rmn.gif|left|200px]]
 
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==A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS==
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The line below this paragraph, containing "STRUCTURE_1rmn", creates the "Structure Box" on the page.
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<StructureSection load='1rmn' size='340' side='right'caption='[[1rmn]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1rmn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RMN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Fluorescence transfer</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmn OCA], [https://pdbe.org/1rmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rmn RCSB], [https://www.ebi.ac.uk/pdbsum/1rmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rmn ProSAT]</span></td></tr>
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{{STRUCTURE_1rmn| PDB=1rmn | SCENE= }}
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</table>
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'''A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS'''
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==See Also==
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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For the understanding of the catalytic function of the RNA hammerhead ribozyme, a three-dimensional model is essential but neither a crystal nor a solution structure has been available. Fluorescence resonance energy transfer (FRET) was used to study the structure of the ribozyme in solution in order to establish the relative spatial orientation of the three constituent Watson-Crick base-paired helical segments. Synthetic constructs were labeled with the fluorescence donor (5-carboxyfluorescein) and acceptor (5-carboxytetramethylrhodamine) located at the ends of the strands constituting the ribozyme molecule. The acceptor helix in helix pairs I and III and in II and III was varied in length from 5 to 11 and 5 to 9 base pairs, respectively, and the FRET efficiencies were determined and correlated with a reference set of labeled RNA duplexes. The FRET efficiencies were predicted on the basis of vector algebra analysis, as a function of the relative helical orientations in the ribozyme constructs, and compared with experimental values. The data were consistent with a Y-shaped arrangement of the ribozyme with helices I and II in close proximity and helix III pointing away. These orientational constraints were used for molecular modeling of a three-dimensional structure of the complete ribozyme.
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[[Category: Large Structures]]
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[[Category: Eckstein F]]
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==About this Structure==
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[[Category: Gohlke C]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMN OCA].
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[[Category: Jovin TM]]
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[[Category: Tuschl T]]
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==Reference==
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[[Category: Westhof E]]
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A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements., Tuschl T, Gohlke C, Jovin TM, Westhof E, Eckstein F, Science. 1994 Nov 4;266(5186):785-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7973630 7973630]
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[[Category: Eckstein, F.]]
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[[Category: Gohlke, C.]]
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[[Category: Jovin, T M.]]
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[[Category: Tuschl, T.]]
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[[Category: Westhof, E.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:40:37 2008''
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A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS

PDB ID 1rmn

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