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| - | [[Image:1rw3.gif|left|200px]] | + | #REDIRECT [[4mh8]] This PDB entry is obsolete and replaced by 4mh8 |
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| - | {{STRUCTURE_1rw3| PDB=1rw3 | SCENE= }}
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| - | '''The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus'''
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| - | ==Overview==
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| - | Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.
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| - | ==About this Structure==
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| - | 1RW3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].
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| - | ==Reference==
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| - | The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus., Das D, Georgiadis MM, Structure. 2004 May;12(5):819-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15130474 15130474]
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| - | [[Category: Moloney murine leukemia virus]]
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| - | [[Category: Single protein]]
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| - | [[Category: Das, D.]]
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| - | [[Category: Georgiadis, M M.]]
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| - | [[Category: Reverse transcriptase]]
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| - | [[Category: Rna and dna dependent dna polymerase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:58:30 2008''
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