1rym

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[[Image:1rym.jpg|left|200px]]
 
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==Structure of the Group II Intron Splicing Factor CRS2==
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The line below this paragraph, containing "STRUCTURE_1rym", creates the "Structure Box" on the page.
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<StructureSection load='1rym' size='340' side='right'caption='[[1rym]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1rym]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rym OCA], [https://pdbe.org/1rym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rym RCSB], [https://www.ebi.ac.uk/pdbsum/1rym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rym ProSAT]</span></td></tr>
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{{STRUCTURE_1rym| PDB=1rym | SCENE= }}
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</table>
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== Function ==
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'''Structure of the Group II Intron Splicing Factor CRS2'''
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[https://www.uniprot.org/uniprot/CRS2_MAIZE CRS2_MAIZE] Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity.<ref>PMID:11179231</ref> <ref>PMID:9090875</ref> <ref>PMID:10481026</ref> <ref>PMID:12881426</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Chloroplast RNA splicing 2 (CRS2) is a nuclear-encoded protein required for the splicing of nine group II introns in maize chloroplasts. CRS2 functions in the context of splicing complexes that include one of two CRS2-associated factors (CAF1 and CAF2). The CRS2-CAF1 and CRS2-CAF2 complexes are required for the splicing of different subsets of CRS2-dependent introns, and they bind tightly and specifically to their genetically defined intron targets in vivo. The CRS2 amino acid sequence is closely related to those of bacterial peptidyl-tRNA hydrolases (PTHs). To identify the structural differences between CRS2 and bacterial PTHs responsible for CRS2's gains of CAF binding and intron splicing functions, we determined the structure of CRS2 by X-ray crystallography. The fold of CRS2 is the same as that of Escherichia coli PTH, but CRS2 has two surfaces that differ from the corresponding surfaces in PTH. One of these is more hydrophobic in CRS2 than in PTH. Site-directed mutagenesis of this surface blocked CRS2-CAF complex formation, indicating that it is the CAF binding site. The CRS2 surface corresponding to the putative tRNA binding face of PTH is considerably more basic than in PTH, suggesting that CRS2 interacts with group II intron substrates via this surface. Both the sequence and the structural context of the amino acid residues essential for peptidyl-tRNA hydrolase activity are conserved in CRS2, yet expression of CRS2 is incapable of rescuing a pth(ts)E.coli strain.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1rym_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1RYM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYM OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rym ConSurf].
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Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces., Ostheimer GJ, Hadjivassiliou H, Kloer DP, Barkan A, Matthews BW, J Mol Biol. 2005 Jan 7;345(1):51-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15567410 15567410]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
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[[Category: Barkan, A.]]
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[[Category: Barkan A]]
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[[Category: Matthews, B W.]]
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[[Category: Matthews BW]]
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[[Category: Ostheimer, G J.]]
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[[Category: Ostheimer GJ]]
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[[Category: Alpha-beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:04:08 2008''
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Current revision

Structure of the Group II Intron Splicing Factor CRS2

PDB ID 1rym

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