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1und

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(New page: 200px<br /> <applet load="1und" size="450" color="white" frame="true" align="right" spinBox="true" caption="1und" /> '''SOLUTION STRUCTURE OF THE HUMAN ADVILLIN C-...)
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[[Image:1und.gif|left|200px]]<br />
 
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<applet load="1und" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1und" />
 
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'''SOLUTION STRUCTURE OF THE HUMAN ADVILLIN C-TERMINAL HEADPIECE SUBDOMAIN'''<br />
 
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==Overview==
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==Solution structure of the human advillin C-terminal headpiece subdomain==
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Headpiece (HP) is a 76-residue F-actin-binding module at the C terminus of, many cytoskeletal proteins. Its 35-residue C-terminal subdomain is one of, the smallest known motifs capable of autonomously adopting a stable, folded structure in the absence of any disulfide bridges, metal ligands, or unnatural amino acids. We report the three-dimensional solution, structures of the C-terminal headpiece subdomains of human villin (HVcHP), and human advillin (HAcHP), determined by two-dimensional 1H-NMR. They, represent the second and third structures of such C-terminal headpiece, subdomains to be elucidated so far. A comparison with the structure of the, chicken villin C-terminal subdomain reveals a high structural, conservation. Both C-terminal subdomains bind specifically to F-actin., Mutagenesis is used to demonstrate the involvement of Trp 64 in the, F-actin-binding surface. The latter residue is part of a conserved, structural feature, in which the surface-exposed indole ring is stacked on, the proline and lysine side chain embedded in a PXWK sequence motif. On, the basis of the structural and mutational data concerning Trp 64 reported, here, the results of a cysteine-scanning mutagenesis study of full, headpiece, and a phage display mutational study of the 69-74 fragment, we, propose a modification of the model, elaborated by Vardar and coworkers, for the binding of headpiece to F-actin.
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<StructureSection load='1und' size='340' side='right'caption='[[1und]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1und]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UND OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UND FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1und FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1und OCA], [https://pdbe.org/1und PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1und RCSB], [https://www.ebi.ac.uk/pdbsum/1und PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1und ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/AVIL_HUMAN AVIL_HUMAN] The disease is caused by variants affecting the gene represented in this entry.
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== Function ==
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[https://www.uniprot.org/uniprot/AVIL_HUMAN AVIL_HUMAN] Ca(2+)-regulated actin-binding protein which plays an important role in actin bundling (PubMed:29058690). May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis. In podocytes, controls lamellipodia formation through the regulation of EGF-induced diacylglycerol generation by PLCE1 and ARP2/3 complex assembly (PubMed:29058690).<ref>PMID:20393563</ref> <ref>PMID:29058690</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/un/1und_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1und ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Headpiece (HP) is a 76-residue F-actin-binding module at the C terminus of many cytoskeletal proteins. Its 35-residue C-terminal subdomain is one of the smallest known motifs capable of autonomously adopting a stable, folded structure in the absence of any disulfide bridges, metal ligands, or unnatural amino acids. We report the three-dimensional solution structures of the C-terminal headpiece subdomains of human villin (HVcHP) and human advillin (HAcHP), determined by two-dimensional 1H-NMR. They represent the second and third structures of such C-terminal headpiece subdomains to be elucidated so far. A comparison with the structure of the chicken villin C-terminal subdomain reveals a high structural conservation. Both C-terminal subdomains bind specifically to F-actin. Mutagenesis is used to demonstrate the involvement of Trp 64 in the F-actin-binding surface. The latter residue is part of a conserved structural feature, in which the surface-exposed indole ring is stacked on the proline and lysine side chain embedded in a PXWK sequence motif. On the basis of the structural and mutational data concerning Trp 64 reported here, the results of a cysteine-scanning mutagenesis study of full headpiece, and a phage display mutational study of the 69-74 fragment, we propose a modification of the model, elaborated by Vardar and coworkers, for the binding of headpiece to F-actin.
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==About this Structure==
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Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements.,Vermeulen W, Vanhaesebrouck P, Van Troys M, Verschueren M, Fant F, Goethals M, Ampe C, Martins JC, Borremans FA Protein Sci. 2004 May;13(5):1276-87. PMID:15096633<ref>PMID:15096633</ref>
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1UND is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UND OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements., Vermeulen W, Vanhaesebrouck P, Van Troys M, Verschueren M, Fant F, Goethals M, Ampe C, Martins JC, Borremans FA, Protein Sci. 2004 May;13(5):1276-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15096633 15096633]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1und" style="background-color:#fffaf0;"></div>
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[[Category: Ampe, C.]]
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[[Category: Borremans, F.]]
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[[Category: Fant, F.]]
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[[Category: Martins, J.]]
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[[Category: Troys, M.Van.]]
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[[Category: Vanhaesebrouck, P.]]
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[[Category: Vermeulen, W.]]
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[[Category: Verschueren, M.]]
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[[Category: ACE]]
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[[Category: cytoskeleton]]
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[[Category: f-actin binding]]
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[[Category: headpiece subdomain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:36:46 2007''
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==See Also==
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*[[Villin|Villin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Ampe C]]
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[[Category: Borremans F]]
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[[Category: Fant F]]
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[[Category: Martins J]]
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[[Category: Van Troys M]]
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[[Category: Vanhaesebrouck P]]
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[[Category: Vermeulen W]]
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[[Category: Verschueren M]]

Current revision

Solution structure of the human advillin C-terminal headpiece subdomain

PDB ID 1und

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