1ryy

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[[Image:1ryy.gif|left|200px]]
 
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==Acetobacter turbidans alpha-amino acid ester hydrolase Y206A mutant==
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The line below this paragraph, containing "STRUCTURE_1ryy", creates the "Structure Box" on the page.
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<StructureSection load='1ryy' size='340' side='right'caption='[[1ryy]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ryy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetobacter_pasteurianus Acetobacter pasteurianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ryy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ryy OCA], [https://pdbe.org/1ryy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ryy RCSB], [https://www.ebi.ac.uk/pdbsum/1ryy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ryy ProSAT]</span></td></tr>
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{{STRUCTURE_1ryy| PDB=1ryy | SCENE= }}
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</table>
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== Function ==
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'''Acetobacter turbidans alpha-amino acid ester hydrolase Y206A mutant'''
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[https://www.uniprot.org/uniprot/Q8VRK8_ACEPA Q8VRK8_ACEPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The alpha-amino acid ester hydrolase (AEH) from Acetobacter turbidans is a bacterial enzyme catalyzing the hydrolysis and synthesis of beta-lactam antibiotics. The crystal structures of the native enzyme, both unliganded and in complex with the hydrolysis product D-phenylglycine are reported, as well as the structures of an inactive mutant (S205A) complexed with the substrate ampicillin, and an active site mutant (Y206A) with an increased tendency to catalyze antibiotic production rather than hydrolysis. The structure of the native enzyme shows an acyl binding pocket, in which D-phenylglycine binds, and an additional space that is large enough to accommodate the beta-lactam moiety of an antibiotic. In the S205A mutant, ampicillin binds in this pocket in a non-productive manner, making extensive contacts with the side chain of Tyr(112), which also participates in oxyanion hole formation. In the Y206A mutant, the Tyr(112) side chain has moved with its hydroxyl group toward the catalytic serine. Because this changes the properties of the beta-lactam binding site, this could explain the increased beta-lactam transferase activity of this mutant.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1ryy_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1RYY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acetobacter_pasteurianus Acetobacter pasteurianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYY OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ryy ConSurf].
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Acetobacter turbidans alpha-amino acid ester hydrolase: how a single mutation improves an antibiotic-producing enzyme., Barends TR, Polderman-Tijmes JJ, Jekel PA, Williams C, Wybenga G, Janssen DB, Dijkstra BW, J Biol Chem. 2006 Mar 3;281(9):5804-10. Epub 2005 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16377627 16377627]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Acetobacter pasteurianus]]
[[Category: Acetobacter pasteurianus]]
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[[Category: Alpha-amino-acid esterase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Barends TRM]]
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[[Category: Barends, T R.M.]]
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[[Category: Alpha/beta hydrolase fold]]
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[[Category: Jellyroll fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:05:06 2008''
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Current revision

Acetobacter turbidans alpha-amino acid ester hydrolase Y206A mutant

PDB ID 1ryy

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