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1urn

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(New page: 200px<br /> <applet load="1urn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1urn, resolution 1.920&Aring;" /> '''U1A MUTANT/RNA COM...)
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[[Image:1urn.gif|left|200px]]<br />
 
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<applet load="1urn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1urn, resolution 1.920&Aring;" />
 
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'''U1A MUTANT/RNA COMPLEX + GLYCEROL'''<br />
 
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==Overview==
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==U1A MUTANT/RNA COMPLEX + GLYCEROL==
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The crystal structure of the RNA-binding domain of the small nuclear, ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been, determined at 1.92 A resolution. The ten-nucleotide RNA loop binds to the, surface of the beta-sheet as an open structure, and the AUUGCAC sequence, of the loop interacts extensively with the conserved RNP1 and RNP2 motifs, and the C-terminal extension of the RNP domain. These interactions include, stacking of RNA bases with aromatic side chains of proteins and many, direct and water-mediated hydrogen bonds. The structure reveals the, stereochemical basis for sequence-specific RNA recognition by the RNP, domain.
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<StructureSection load='1urn' size='340' side='right'caption='[[1urn]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1urn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1URN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1URN FirstGlance]. <br>
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1URN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1URN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1urn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1urn OCA], [https://pdbe.org/1urn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1urn RCSB], [https://www.ebi.ac.uk/pdbsum/1urn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1urn ProSAT]</span></td></tr>
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Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin., Oubridge C, Ito N, Evans PR, Teo CH, Nagai K, Nature. 1994 Dec 1;372(6505):432-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7984237 7984237]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNRPA_HUMAN SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.<ref>PMID:9848648</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ur/1urn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1urn ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Evans, P.R.]]
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[[Category: Evans PR]]
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[[Category: Ito, N.]]
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[[Category: Ito N]]
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[[Category: Nagai, K.]]
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[[Category: Nagai K]]
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[[Category: Oubridge, C.]]
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[[Category: Oubridge C]]
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[[Category: Teo, C.H.]]
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[[Category: Teo C-H]]
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[[Category: CL]]
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[[Category: GOL]]
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[[Category: hairpin loop]]
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[[Category: overhanging base]]
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[[Category: protein-rna complex]]
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[[Category: single strand]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:37:56 2007''
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Current revision

U1A MUTANT/RNA COMPLEX + GLYCEROL

PDB ID 1urn

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