1s28

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[[Image:1s28.gif|left|200px]]
 
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==Crystal Structure of AvrPphF ORF1, the Chaperone for the Type III Effector AvrPphF ORF2 from P. syringae==
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The line below this paragraph, containing "STRUCTURE_1s28", creates the "Structure Box" on the page.
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<StructureSection load='1s28' size='340' side='right'caption='[[1s28]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_savastanoi_pv._phaseolicola Pseudomonas savastanoi pv. phaseolicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S28 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1s28| PDB=1s28 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s28 OCA], [https://pdbe.org/1s28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s28 RCSB], [https://www.ebi.ac.uk/pdbsum/1s28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s28 ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of AvrPphF ORF1, the Chaperone for the Type III Effector AvrPphF ORF2 from P. syringae'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q9K2L2_PSESH Q9K2L2_PSESH]
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<div style="background-color:#fffaf0;">
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==Overview==
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== Publication Abstract from PubMed ==
The avrPphF locus from Pseudomonas syringae pv. phaseolicola, the causative agent of bean halo-blight disease, encodes proteins which either enhance virulence on susceptible hosts or elicit defense responses on hosts carrying the R1 resistance gene. Here we present the crystal structures of the two proteins from the avrPphF operon. The structure of AvrPphF ORF1 is strikingly reminiscent of type III chaperones from bacterial pathogens of animals, indicating structural conservation of these specialized chaperones, despite high sequence divergence. The AvrPphF ORF2 effector adopts a novel "mushroom"-like structure containing "head" and "stalk" subdomains. The head subdomain possesses limited structural homology to the catalytic domain of bacterial ADP-ribosyltransferases (ADP-RTs), though no ADP-RT activity was detected for AvrPphF ORF2 in standard assays. Nonetheless, this structural similarity identified two clusters of conserved surface-exposed residues important for both virulence mediated by AvrPphF ORF2 and recognition of this effector by bean plants expressing the R1 resistance gene.
The avrPphF locus from Pseudomonas syringae pv. phaseolicola, the causative agent of bean halo-blight disease, encodes proteins which either enhance virulence on susceptible hosts or elicit defense responses on hosts carrying the R1 resistance gene. Here we present the crystal structures of the two proteins from the avrPphF operon. The structure of AvrPphF ORF1 is strikingly reminiscent of type III chaperones from bacterial pathogens of animals, indicating structural conservation of these specialized chaperones, despite high sequence divergence. The AvrPphF ORF2 effector adopts a novel "mushroom"-like structure containing "head" and "stalk" subdomains. The head subdomain possesses limited structural homology to the catalytic domain of bacterial ADP-ribosyltransferases (ADP-RTs), though no ADP-RT activity was detected for AvrPphF ORF2 in standard assays. Nonetheless, this structural similarity identified two clusters of conserved surface-exposed residues important for both virulence mediated by AvrPphF ORF2 and recognition of this effector by bean plants expressing the R1 resistance gene.
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==About this Structure==
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Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis.,Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J Structure. 2004 Sep;12(9):1669-81. PMID:15341731<ref>PMID:15341731</ref>
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1S28 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._phaseolicola Pseudomonas syringae pv. phaseolicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S28 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis., Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J, Structure. 2004 Sep;12(9):1669-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341731 15341731]
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</div>
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[[Category: Pseudomonas syringae pv. phaseolicola]]
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<div class="pdbe-citations 1s28" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Betts, L.]]
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<references/>
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[[Category: Chang, J H.]]
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__TOC__
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[[Category: Dangl, J L.]]
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</StructureSection>
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[[Category: Desveaux, D.]]
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[[Category: Large Structures]]
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[[Category: Grant, S R.]]
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[[Category: Pseudomonas savastanoi pv. phaseolicola]]
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[[Category: Nimchuk, Z.]]
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[[Category: Betts L]]
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[[Category: Singer, A U.]]
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[[Category: Chang JH]]
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[[Category: Sondek, J.]]
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[[Category: Dangl JL]]
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[[Category: Type iii chaperone]]
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[[Category: Desveaux D]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:12:19 2008''
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[[Category: Grant SR]]
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[[Category: Nimchuk Z]]
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[[Category: Singer AU]]
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[[Category: Sondek J]]

Current revision

Crystal Structure of AvrPphF ORF1, the Chaperone for the Type III Effector AvrPphF ORF2 from P. syringae

PDB ID 1s28

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