|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1uw2.gif|left|200px]]<br /> | + | #REDIRECT [[2vrd]] This PDB entry is obsolete and replaced by 2vrd |
| - | <applet load="1uw2" size="450" color="white" frame="true" align="right" spinBox="true"
| + | |
| - | caption="1uw2" />
| + | |
| - | '''THE STRUCTURE OF THE ZINC FINGER FROM THE HUMAN SPLICEOSOMAL PROTEIN U1C'''<br />
| + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | The spliceosomal U1C protein is critical to the initiation and regulation, of precursor messenger RNA (pre-mRNA) splicing, as part of the U1 small, nuclear ribonucleoprotein particle (snRNP). We have produced full-length, and 61 residue constructs of human U1C in soluble form in Escherichia, coli. Atomic absorption spectroscopy and mass spectrometry show that both, constructs contain one Zn atom and are monomeric. Gelmobility-shift assays, showed that one molecule of recombinant U1C, either full-length or 61, residue construct, can be incorporated into the U1 snRNP core domain in, the presence of U1 70k. This result is in perfect agreement with the, previous experiment with U1C isolated from the HeLa U1 snRNP showing that, the recombinant U1C is functionally active. We have determined the, solution structure of the N-terminal 61 residue construct of U1C by NMR. A, Cys(2)His(2)-type zinc finger, distinct from the TFIIIA-type, is extended, at its C terminus by two additional helices. The two Zn-coordinating, histidine residues are separated by a five residue loop. The conserved, basic residues in the first two helices and the intervening loop may be, involved in RNA binding. The opposite beta-sheet face with two, surface-exposed Tyr residues may be involved in protein contacts. Both the, full-length and 61 residue constructs of human U1C fail to bind RNA, containing the 5' splice site sequence, in contrast to what has been, reported for the Saccharomyces cerevisiae orthologue.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1UW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UW2 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | The structure and biochemical properties of the human spliceosomal protein U1C., Muto Y, Pomeranz Krummel D, Oubridge C, Hernandez H, Robinson CV, Neuhaus D, Nagai K, J Mol Biol. 2004 Jul 30;341(1):185-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15312772 15312772]
| + | |
| - | [[Category: Homo sapiens]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Hernandez, H.]]
| + | |
| - | [[Category: Krummel, D.Pomeranz.]]
| + | |
| - | [[Category: Muto, Y.]]
| + | |
| - | [[Category: Nagai, K.]]
| + | |
| - | [[Category: Neuhaus, D.]]
| + | |
| - | [[Category: Oubridge, C.]]
| + | |
| - | [[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
| + | |
| - | [[Category: Robinson, C.]]
| + | |
| - | [[Category: ZN]]
| + | |
| - | [[Category: nuclear protein]]
| + | |
| - | [[Category: riken structural genomics/proteomics initiative]]
| + | |
| - | [[Category: rna-binding protein]]
| + | |
| - | [[Category: rsgi]]
| + | |
| - | [[Category: spliceosomal protein]]
| + | |
| - | [[Category: structural genomics]]
| + | |
| - | [[Category: u1 snrnp]]
| + | |
| - | [[Category: zinc finger]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:39:15 2007''
| + | |
